Department of Biotechnology and Microbiology of Foods, Institute of Food Science Research (CIAL) (CSIC-UAM), Nicolás Cabrera 9, 28049 Madrid, Spain.
Department of Production and Characterization of Novel Foods, Institute of Food Science Research (CIAL) (CSIC-UAM), Nicolás Cabrera 9, 28049 Madrid, Spain.
Molecules. 2023 Jun 9;28(12):4675. doi: 10.3390/molecules28124675.
High-pressure homogenization (HPH) is an emerging technology for obtaining physical and microbial stability of plant-based milks, but there is little information on the effects of this technology on the phytochemical components of the processed plant food beverage and during its cold storage. The effect of three selected HPH treatments (180 MPa/25 °C, 150 MPa/55 °C, and 50 MPa/75 °C) and pasteurization (PAS) (63 °C, 20 min) on minor lipid constituents, total proteins, phenolic compounds, antioxidant capacity, and essential minerals of Brazil nut beverage (BNB) were studied. Additionally, the study of the possible changes in these constituents was carried out during cold storage at 5 °C for 21 days. The fatty acid profile (dominated by oleic acid and linoleic acid), free fatty acid content, protein, and essential minerals (notable source of Se and Cu) of the processed BNB remained almost stable to treatments (HPH and PAS). Specifically, reductions in squalene (22.7 to 26.4%) and γ-γ-tocopherol (28.4 to 36%) were observed in beverages processed via both non-thermal HPH and thermal PAS, but β-sitosterol remained unchanged. Total phenolics were reduced (24 to 30%) after both treatments, a factor that influenced the observed antioxidant capacity. The studied individual phenolics in BNB were gallic acid, catechin, epicatechin, catechin gallate, and ellagic acid, being the most abundant compounds. During cold storage (5 °C) up to 21 days, changes in the content of phytochemicals, minerals, and total proteins were not noticeable for any treated beverages, and no lipolysis processes were promoted. Therefore, after the application of HPH processing, Brazil nut beverage (BNB) maintained almost unaltered levels of bioactive compounds, essential minerals, total protein, and oxidative stability, remarkable characteristics for its potential development as a functional food.
高压均质(HPH)是一种获得植物奶物理和微生物稳定性的新兴技术,但关于该技术对加工植物食品饮料的植物化学成分的影响以及在其冷藏过程中的影响的信息很少。研究了三种选定的 HPH 处理(180 MPa/25°C、150 MPa/55°C 和 50 MPa/75°C)和巴氏杀菌(PAS)(63°C,20 分钟)对巴西坚果饮料(BNB)中微量脂质成分、总蛋白、酚类化合物、抗氧化能力和必需矿物质的影响。此外,还在 5°C 下冷藏 21 天期间研究了这些成分可能发生的变化。加工后的 BNB 的脂肪酸谱(以油酸和亚油酸为主)、游离脂肪酸含量、蛋白质和必需矿物质(硒和铜的重要来源)在处理(HPH 和 PAS)中几乎保持稳定。具体而言,通过非热 HPH 和热 PAS 处理的饮料中观察到角鲨烯(22.7%至 26.4%)和γ-γ-生育酚(28.4%至 36%)的减少,但β-谷甾醇保持不变。两种处理后总酚含量均降低(24%至 30%),这一因素影响了观察到的抗氧化能力。在 BNB 中研究的单个酚类化合物是没食子酸、儿茶素、表儿茶素、儿茶素没食子酸酯和鞣花酸,它们是含量最丰富的化合物。在冷藏(5°C)期间长达 21 天,任何处理的饮料中的植物化学物质、矿物质和总蛋白质含量均无明显变化,也没有促进脂肪水解过程。因此,在应用 HPH 处理后,巴西坚果饮料(BNB)保持了几乎不变的生物活性化合物、必需矿物质、总蛋白质和氧化稳定性水平,这是其作为功能性食品开发的显著特征。