Institute of Marine Research (HI), P.O. Box 1870 Nordnes, N-5817 Bergen, Norway.
Department of Chemistry, University of Bergen, Allégaten 41, N-5007 Bergen, Norway.
Molecules. 2023 Jun 13;28(12):4728. doi: 10.3390/molecules28124728.
A simple and rapid method for the extraction of D-series resolvins (RvD1, RvD2, RvD3, RvD4, RvD5) released into Leibovitz's L-15 complete medium by head kidney cells from Atlantic salmon and the further determination of liquid chromatography triple quadrupole mass spectrometry is proposed. A three-level factorial design was proposed to select the optimal concentrations of internal standards that were used in the evaluation of the performance parameters, such as linear range (0.1-50 ng mL), limits of detection and quantification (0.05 and 0.1 ng mL, respectively), and recovery values ranging from 96.9 to 99.8%. The optimized method was used to determine the stimulated production of resolvins by head kidney cells exposed to docosahexaenoic acid, and the results indicated that it is possible that the production was controlled by circadian responses.
提出了一种从大西洋三文鱼头肾细胞中提取莱氏培养液 L-15 中释放的 D 系列 resolvins（RvD1、RvD2、RvD3、RvD4、RvD5）的简单、快速方法，并进一步采用液相色谱三重四极杆质谱法进行了测定。采用三因素析因设计法选择了内标物的最佳浓度，用于评价性能参数，如线性范围（0.1-50ng/mL）、检测限和定量限（分别为 0.05 和 0.1ng/mL）以及回收率（96.9-99.8%）。优化后的方法用于测定头肾细胞暴露于二十二碳六烯酸后 resolvins 的刺激产量，结果表明，这种产量可能受昼夜节律反应的控制。
