Laboratory of Analytical Chemistry, School of Chemistry, Faculty of Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
Molecules. 2023 Jun 17;28(12):4831. doi: 10.3390/molecules28124831.
This study reports a new approach for the determination of copper ions in water samples that exploits the complexation reaction with diethyldithiocarbamate (DDTC) and uses widely available imaging devices (i.e., flatbed scanners or smartphones) as detectors. Specifically, the proposed approach is based on the ability of DDTC to bind to copper ions and form a stable Cu-DDTC complex with a distinctive yellow color detected with the camera of a smartphone in a 96-well plate. The color intensity of the formed complex is linearly proportional to the concentration of copper ions, resulting in its accurate colorimetric determination. The proposed analytical procedure for the determination of Cu was easy to perform, rapid, and applicable with inexpensive and commercially available materials and reagents. Many parameters related to such an analytical determination were optimized, and a study of interfering ions present in the water samples was also carried out. Additionally, even low copper levels could be noticed by the naked eye. The assay performed was successfully applied to the determination of Cu in river, tap, and bottled water samples with detection limits as low as 1.4 µM, good recoveries (89.0-109.6%), adequate reproducibility (0.6-6.1%), and high selectivity over other ions present in the water samples.
本研究报告了一种新的方法，用于测定水样中的铜离子，该方法利用了与二乙基二硫代氨基甲酸盐（DDTC）的络合反应，并使用广泛可用的成像设备（即平板扫描仪或智能手机）作为检测器。具体来说，该方法基于 DDTC 与铜离子结合并形成稳定的 Cu-DDTC 络合物的能力，该络合物具有独特的黄色，可在智能手机的相机中在 96 孔板中检测到。形成的络合物的颜色强度与铜离子的浓度呈线性比例，从而可以准确地比色测定。所提出的用于测定 Cu 的分析程序易于执行，快速，并且可以使用廉价且市售的材料和试剂进行。对这种分析测定相关的许多参数进行了优化，并对水样中存在的干扰离子进行了研究。此外，即使是低浓度的铜也可以用肉眼察觉。该测定方法成功地应用于河水、自来水和瓶装水中 Cu 的测定，检测限低至 1.4 µM，回收率良好（89.0-109.6%），重现性良好（0.6-6.1%），对水样中存在的其他离子具有高选择性。
