Prpić Jelena, Lojkić Ivana, Keros Tomislav, Krešić Nina, Jemeršić Lorena
Croatian Veterinary Institute, Savska cesta 143, 10000 Zagreb, Croatia.
Pathogens. 2023 Jun 15;12(6):833. doi: 10.3390/pathogens12060833.
The canine distemper virus (CDV), a paramyxovirus that is closely related to the human measles virus and rinderpest virus of cattle, is a highly contagious viral disease in dogs and wild carnivores worldwide. CDV represents a serious threat to domestic and wild animals, especially to the conservation of endangered wild carnivores. Our study aims to investigate the occurrence of CDV in free-living wild canines in Croatia. For this purpose, 176 red foxes and 24 jackal brain samples collected in the frame of the active surveillance of rabies during winter 2021/2022 were tested. This study provided the first comprehensive overview of the prevalence and spatial distribution of CDV in the wildlife of Croatia, including the molecular phylogenetic analysis of the H gene sequence of field CDV strains circulating in red fox and jackal populations of Croatia. The molecular characterization of hemagglutinin gene genomic regions confirmed the phylogenetic clustering of obtained sequences into the Europa 1 genotype. The obtained CDV red fox sequences were mutually very similar (97.60%). This study indicates the high genetic similarity of Croatian CDV red fox sequences and CDV red fox sequences from Italy and Germany, badger sequences from Germany, polecat sequences from Hungary, and dog sequences from Hungary and Germany.
犬瘟热病毒（CDV）是一种副粘病毒，与人类麻疹病毒和牛瘟病毒密切相关，是全球犬类和野生食肉动物中一种高度传染性的病毒性疾病。CDV对家养动物和野生动物构成严重威胁，尤其是对濒危野生食肉动物的保护。我们的研究旨在调查克罗地亚自由生活的野生犬科动物中CDV的发生情况。为此，对在2021/2022年冬季狂犬病主动监测框架下收集的176份赤狐和24份豺脑样本进行了检测。这项研究首次全面概述了克罗地亚野生动物中CDV的流行情况和空间分布，包括对克罗地亚赤狐和豺种群中传播的野外CDV毒株H基因序列的分子系统发育分析。血凝素基因基因组区域的分子特征证实，获得的序列在系统发育上聚类为Europa 1基因型。获得的CDV赤狐序列彼此非常相似（97.60%）。这项研究表明，克罗地亚CDV赤狐序列与来自意大利和德国的CDV赤狐序列、来自德国的獾序列、来自匈牙利的鸡貂序列以及来自匈牙利和德国的犬序列具有高度的遗传相似性。
