Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China.
Nutrients. 2023 Jun 9;15(12):2697. doi: 10.3390/nu15122697.
essential oil (LEO) has been reported to have antibacterial and anti-inflammatory activities, but its protective effect in the intestine remains unknown. In this study, we researched the protective effects of LEO in relation to intestinal inflammation induced by K99. The mice were pretreated with 300, 600, and 1200 mg/kg LEO and then stimulated with K99. The results showed that K99 caused immune organ responses, intestinal tissue injury, and inflammation. LEO pretreatment dose-dependently alleviated these changes by maintaining a low index in the thymus and spleen and producing a high content of immunoglobulin A, G, and M (IgA, IgG, and IgM) and low content of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6). Intestinal integrity as a consequence of the LEO pretreatment may be related to the high mRNA expression of intestinal trefoil factor (ITF) and the low mRNA expression of transforming growth factor-β1 (TGF-β1). Conclusively, an LEO pretreatment can alleviate K99-induced diarrhea, immune organ response, and body inflammation in mice by reducing the levels of inflammatory cytokines and improving the levels of immunoglobulin, and the intestinal integrity remained highest when maintaining the high mRNA expression of ITF and keeping the mRNA expression of TGF-β1 low in the intestinal tissue.
精油（LEO）已被报道具有抗菌和抗炎活性，但它在肠道中的保护作用尚不清楚。在这项研究中，我们研究了 LEO 对 K99 诱导的肠道炎症的保护作用。小鼠先用 300、600 和 1200mg/kg 的 LEO 预处理，然后用 K99 刺激。结果表明，K99 引起免疫器官反应、肠道组织损伤和炎症。LEO 预处理通过维持胸腺和脾脏的低指数，产生高含量的免疫球蛋白 A、G 和 M（IgA、IgG 和 IgM）和低含量的肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6），从而剂量依赖性地缓解了这些变化。LEO 预处理对肠道完整性的影响可能与肠道三叶因子（ITF）的高 mRNA 表达和转化生长因子-β1（TGF-β1）的低 mRNA 表达有关。总之，LEO 预处理可以通过降低炎症细胞因子水平和提高免疫球蛋白水平来减轻 K99 诱导的腹泻、免疫器官反应和机体炎症，在肠道组织中保持 ITF 的高 mRNA 表达和 TGF-β1 的低 mRNA 表达时，肠道完整性保持最高。
