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通过电穿孔法评估导入人乳腺癌细胞MCF-7/WT和MCF-7/DOX中的钙离子的抗癌活性。

Evaluation of the Anticancer Activity of Calcium Ions Introduced into Human Breast Adenocarcinoma Cells MCF-7/WT and MCF-7/DOX by Electroporation.

作者信息

Bieżuńska-Kusiak Katarzyna, Kulbacka Julita, Choromańska Anna, Rembiałkowska Nina, Michel Olga, Saczko Jolanta

机构信息

Department of Molecular and Cellular Biology, Wroclaw Medical University, Borowska 211A, 50-556 Wroclaw, Poland.

Department of Immunology, State Research Institute Centre for Innovative Medicine, Santariškių 5, 08410 Vilnius, Lithuania.

出版信息

Pharmaceuticals (Basel). 2023 May 30;16(6):809. doi: 10.3390/ph16060809.

DOI:10.3390/ph16060809
PMID:37375757
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10304440/
Abstract

Breast cancer ranks among the top three most common malignant neoplasms in Poland. The use of calcium ion-assisted electroporation is an alternative approach to the classic treatment of this disease. The studies conducted in recent years confirm the effectiveness of electroporation with calcium ions. Electroporation is a method that uses short electrical pulses to create transitional pores in the cell membrane to allow the penetration of certain drugs. The aim of this study was to investigate the antitumor effects of electroporation alone and calcium ion-assisted electroporation on human mammary adenocarcinoma cells that are sensitive (MCF-7/WT) and resistant to doxorubicin (MCF-7/DOX). The cell viability was assessed using independent tests: MTT and SRB. The type of cell death after the applied therapy was determined by TUNEL and flow cytometry (FACS) methods. The expression of Cav3.1 and Cav3.2 proteins of T-type voltage-gated calcium channels was assessed by immunocytochemistry, and changes in the morphology of CaEP-treated cells were visualized using a holotomographic microscope. The obtained results confirmed the effectiveness of the investigated therapeutic method. The results of the work constitute a good basis for planning research at the in vivo level and in the future to develop a more effective and safer method of breast cancer treatment for patients.

摘要

乳腺癌位列波兰最常见的三大恶性肿瘤之中。钙离子辅助电穿孔疗法是治疗该疾病的一种替代传统疗法的方法。近年来开展的研究证实了钙离子电穿孔的有效性。电穿孔是一种利用短电脉冲在细胞膜上形成过渡性孔隙，以使某些药物得以渗透的方法。本研究的目的是探究单独电穿孔以及钙离子辅助电穿孔对人乳腺腺癌细胞（对阿霉素敏感的MCF-7/WT和对阿霉素耐药的MCF-7/DOX）的抗肿瘤作用。使用独立测试MTT和SRB评估细胞活力。通过TUNEL和流式细胞术（FACS）方法确定所应用疗法后的细胞死亡类型。通过免疫细胞化学评估T型电压门控钙通道的Cav3.1和Cav3.2蛋白的表达，并使用全息断层显微镜观察经钙离子电穿孔处理的细胞的形态变化。所获得的结果证实了所研究治疗方法的有效性。该研究结果为在体内水平进行研究以及未来为患者开发更有效、更安全的乳腺癌治疗方法奠定了良好基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62d1/10304440/b6f0900a87fb/pharmaceuticals-16-00809-g010.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62d1/10304440/7bb4a23eabef/pharmaceuticals-16-00809-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62d1/10304440/c56348441f8f/pharmaceuticals-16-00809-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62d1/10304440/3d9635bdaaa3/pharmaceuticals-16-00809-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62d1/10304440/0f65e3f74a85/pharmaceuticals-16-00809-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62d1/10304440/7bb4a23eabef/pharmaceuticals-16-00809-g008.jpg
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