Taboada Jorge, González-Gordo Salvador, Muñoz-Vargas María A, Palma José M, Corpas Francisco J
Department of Stress, Development and Signaling in Plants, Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Estación Experimental del Zaidín (Spanish National Research Council, CSIC), C/Profesor Albareda, 1, 18008 Granada, Spain.
Plants (Basel). 2023 Jun 17;12(12):2353. doi: 10.3390/plants12122353.
NADPH is an indispensable cofactor in a wide range of physiological processes that is generated by a family of NADPH dehydrogenases, of which the NADP-dependent malic enzyme (NADP-ME) is a member. Pepper ( L.) fruit is a horticultural product consumed worldwide that has great nutritional and economic relevance. Besides the phenotypical changes that pepper fruit undergoes during ripening, there are many associated modifications at transcriptomic, proteome, biochemical and metabolic levels. Nitric oxide (NO) is a recognized signal molecule with regulatory functions in diverse plant processes. To our knowledge, there is very scarce information about the number of genes encoding for NADP-ME in pepper plants and their expression during the ripening of sweet pepper fruit. Using a data mining approach to evaluate the pepper plant genome and fruit transcriptome (RNA-seq), five genes were identified, and four of them, namely - to -, were expressed in fruit. The time course expression analysis of these genes during different fruit ripening stages, including green immature (G), breaking point (BP) and red ripe (R), showed that they were differentially modulated. Thus, while - and - were upregulated, and were downregulated. Exogenous NO treatment of fruit triggered the downregulation of . We obtained a 50-75% ammonium-sulfate-enriched protein fraction containing CaNADP-ME enzyme activity, and this was assayed via non-denaturing polyacrylamide gel electrophoresis (PAGE). The results allow us to identify four isozymes designated from CaNADP-ME I to CaNADP-ME IV. Taken together, the data provide new pieces of information on the CaNADP-ME system with the identification of five genes and how the four genes expressed in pepper fruits are modulated during ripening and exogenous NO gas treatment.
烟酰胺腺嘌呤二核苷酸磷酸（NADPH）是一系列生理过程中不可或缺的辅助因子，由NADPH脱氢酶家族产生，其中依赖烟酰胺腺嘌呤二核苷酸磷酸的苹果酸酶（NADP-ME）是该家族的一员。辣椒（L.）果实是一种在全球范围内消费的园艺产品，具有重要的营养和经济价值。除了辣椒果实在成熟过程中经历的表型变化外，在转录组、蛋白质组、生化和代谢水平上还有许多相关的修饰。一氧化氮（NO）是一种公认的信号分子，在多种植物过程中具有调节功能。据我们所知，关于辣椒植株中编码NADP-ME的基因数量及其在甜椒果实成熟过程中的表达情况，信息非常匮乏。通过数据挖掘方法评估辣椒植株基因组和果实转录组（RNA测序），鉴定出了五个基因，其中四个基因，即-至-，在果实中表达。对这些基因在不同果实成熟阶段，包括绿色未成熟（G）、转折点（BP）和红色成熟（R）的时间进程表达分析表明，它们受到不同的调控。因此，虽然-和-上调，但和下调。果实的外源NO处理引发了的下调。我们获得了一个含有CaNADP-ME酶活性的50-75%硫酸铵富集蛋白组分，并通过非变性聚丙烯酰胺凝胶电泳（PAGE）对其进行了分析。结果使我们能够鉴定出四种同工酶，命名为CaNADP-ME I至CaNADP-ME IV。综上所述，这些数据提供了关于CaNADP-ME系统的新信息，包括五个基因的鉴定以及辣椒果实中表达的四个基因在成熟和外源NO气体处理过程中是如何被调控的。
