Key Laboratory of Livestock Disease Prevention of Guangdong Province, Key Laboratory of Avian Influenza and Other Major Poultry Diseases Prevention and Control, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming 650224, China.
Viruses. 2023 May 28;15(6):1263. doi: 10.3390/v15061263.
Epizootic hemorrhagic disease (EHD) is an infectious viral disease caused by epizootic hemorrhagic disease virus (EHDV) and EHDV frequently circulates in wild and domestic ruminants. Sporadic outbreaks of EHD have caused thousands of deaths and stillbirths on cattle farms. However, not much is known about the circulating status of EHDV in Guangdong, southern China. To estimate the seroprevalence of EHDV in Guangdong province, 2886 cattle serum samples were collected from 2013 to 2017 and tested for antibodies against EHDV using a competitive ELISA. The overall seroprevalence of EHDV reached 57.87% and was highest in autumn (75.34%). A subset of positive samples were serotyped by a serum neutralization test, showing that EHDV serotypes 1 and 5-8 were circulating in Guangdong. In addition, EHDV prevalence always peaked in autumn, while eastern Guangdong had the highest EHDV seropositivity over the five-year period, displaying apparent temporal-spatial distribution of EHDV prevalence. A binary logistic model analysis indicated a significant association between cattle with BTV infections and seroprevalence of EHDV (OR = 1.70, < 0.001). The co-infection of different serotypes of EHDV and BTV raises a high risk of potential genomic reassortment and is likely to pose a significant threat to cattle, thus urging more surveillance to monitor their circulating dynamics in China.
动物流行病出血病（EHD）是一种由动物流行病出血病病毒（EHDV）引起的传染性病毒病，EHDV 经常在野生和家养反刍动物中传播。EHD 的零星爆发已导致数千头奶牛在牛场死亡和流产。然而，对于中国南方广东省 EHDV 的循环状况，人们知之甚少。为了估计广东省 EHDV 的血清流行率，我们从 2013 年到 2017 年收集了 2886 份牛血清样本，并使用竞争 ELISA 检测了针对 EHDV 的抗体。EHDV 的总体血清流行率达到 57.87%，秋季最高（75.34%）。通过血清中和试验对一部分阳性样本进行了血清分型，结果表明 EHDV 血清型 1 和 5-8 在中国广东省流行。此外，EHDV 的流行率总是在秋季达到峰值，而在五年期间，广东省东部的 EHDV 血清阳性率最高，显示出 EHDV 流行率的明显时空分布。二项逻辑模型分析表明，牛感染 BTV 与 EHDV 血清阳性率之间存在显著关联（OR = 1.70，<0.001）。EHDV 和 BTV 的不同血清型的共同感染增加了潜在基因组重配的高风险，这可能对牛构成重大威胁，因此需要加强监测以监测其在中国的循环动态。
