Studio Ace Enterprise and Pretties, Shimosueyoshi 5- 5- 2, Tsurumi, Kanagawa, 230-0012, Japan.
Graduate School of Integrative Science and Engineering, Tokyo City University, Tamadzutsumi, Setagata, Tokyo, 158-8557, Japan.
J Chem Ecol. 2023 Aug;49(7-8):397-407. doi: 10.1007/s10886-023-01433-2. Epub 2023 Jun 28.
An electroantennogram (EAG) technique compared the antennal olfactory responses by both sexes of eight Japanese Papilio species with known host plants in laboratory experiments. Papilio species were collected from Honshû and Kyûshû (Japanese islands). The behavioral responses to volatile leaf substances from Citrus deliciosa, Zanthoxylum ailanthoides, Phellodendron amurense, Orixa japonica, and Foeniculum vulgare were examined in laboratory experiments. Individual EAG reactions were recorded. The results were very similar to the empirical field observations. The electrophysiological results of both sexes showed that the volatile substances released from non-preferred plants mainly elicited more significant EAG responses than the volatile substances from preferred host plants. Moreover, we performed behavioral experiments using eight female butterflies and their responses to five host plant species. An association between host plant selection behavior and taxonomical classification exists within the Papilio genus. The EAG responses were small when exposed to the plants with high scores in the behavioral experiments. Host plant preference patterns seem to be related to the volatile substances within the host plants. The butterflies responded to Linalool in both the behavioral and electrophysiological experiments.
利用触角电位（EAG）技术，在实验室实验中比较了 8 种具有已知寄主植物的日本凤蝶雌雄两性的触角嗅觉反应。凤蝶物种采集自本州和九州（日本岛屿）。在实验室实验中，研究了柑橘、花椒、黄檗、日本橘和茴香的挥发性叶物质对凤蝶的行为反应。记录了个体 EAG 反应。结果与经验性野外观察非常相似。雌雄两性的电生理结果表明，非首选植物释放的挥发性物质主要引起比首选寄主植物的挥发性物质更显著的 EAG 反应。此外，我们使用 8 只雌性蝴蝶进行了行为实验，并研究了它们对 5 种寄主植物的反应。寄主植物选择行为与凤蝶属的分类学分类之间存在关联。在行为实验中得分较高的植物暴露时，EAG 反应较小。寄主植物偏好模式似乎与寄主植物内的挥发性物质有关。蝴蝶在行为和电生理实验中都对芳樟醇做出了反应。
