Hu Ping, Ren Yueming, Xu Jun, Luo Wanglong, Wang Mengfei, Song Puwen, Guan Yuanyuan, Hu Haiyan, Li Chengwei
College of Life Science and Technology, Henan Engineering Research Center of Crop Genome Editing/Henan International Joint Laboratory of Plant Genetic Improvement and Soil Remediation, Henan Institute of Science and Technology, Xinxiang, China.
College of Life Science and Technology, Henan Engineering Research Center of Crop Genome Editing/Henan International Joint Laboratory of Plant Genetic Improvement and Soil Remediation, Henan Institute of Science and Technology, Xinxiang, China.
Int J Biol Macromol. 2023 Aug 15;246:125526. doi: 10.1016/j.ijbiomac.2023.125526. Epub 2023 Jun 26.
Plant acyl-CoA-binding proteins (ACBPs), which contain the conserved ACB domain, participate in multiple biological processes, however, there are few reports on wheat ACBPs. In this study, the ACBP genes from nine different species were identified comprehensively. The expression patterns of TaACBP genes in multiple tissues and under various biotic stresses were determined by qRT-PCR. The function of selected TaACBP genes was studied by virus-induced gene silencing. A total of 67 ACBPs were identified from five monocotyledonous and four dicotyledonous species and divided into four classes. Tandem duplication analysis of the ACBPs suggested that tandem duplication events occurred in Triticum dicoccoides, but there was no tandem duplication event in wheat ACBP genes. Evolutionary analysis suggested that the TdACBPs may have experienced gene introgression during tetraploid evolution, while TaACBP gene loss events occurred during hexaploid wheat evolution. The expression pattern showed that all the TaACBP genes were expressed, and most of them were responsive to induction by Blumeria graminis f. sp. tritici or Fusarium graminearum. Silencing of TaACBP4A-1 and TaACBP4A-2 increased powdery mildew susceptibility in the common wheat BainongAK58. Furthermore, TaACBP4A-1, which belonged to class III, physically interacted with autophagy-related ubiquitin-like protein TaATG8g in yeast cells. This study provided a valuable reference for further investigations into the functional and molecular mechanisms of the ACBP gene family.
植物酰基辅酶A结合蛋白（ACBPs）含有保守的ACB结构域，参与多种生物学过程，然而，关于小麦ACBPs的报道较少。在本研究中，全面鉴定了来自9个不同物种的ACBP基因。通过qRT-PCR测定了TaACBP基因在多个组织以及各种生物胁迫下的表达模式。通过病毒诱导的基因沉默研究了所选TaACBP基因的功能。从5种单子叶植物和4种双子叶植物中总共鉴定出67个ACBPs，并分为四类。对ACBPs的串联重复分析表明，二粒小麦中发生了串联重复事件，但小麦ACBP基因中没有串联重复事件。进化分析表明，四倍体进化过程中，二粒小麦ACBPs可能经历了基因渗入，而六倍体小麦进化过程中发生了TaACBP基因丢失事件。表达模式显示，所有TaACBP基因均有表达，且大多数基因对小麦白粉病菌或禾谷镰刀菌的诱导有响应。在普通小麦百农AK58中，TaACBP4A-1和TaACBP4A-2基因沉默增加了对白粉病的敏感性。此外，属于III类的TaACBP4A-1在酵母细胞中与自噬相关的泛素样蛋白TaATG8g发生物理相互作用。本研究为进一步研究ACBP基因家族的功能和分子机制提供了有价值的参考。
