Harder M E, Banaszak L J
Biochim Biophys Acta. 1979 Mar 23;552(1):89-102. doi: 10.1016/0005-2736(79)90248-7.
Small angle X-ray data from purified forms of inner or cytoplasmic and outer membranes from Escherichia coli have been obtained and appear to be qualitatively similar. Transitory changes are apparent in the circularly averaged X-ray profiles from inner membranes. Such results could be due to the loss or denaturation of peripheral membrane proteins. Some partially dried forms of outer membrane are partly ordered and produce diffraction patterns which support an underlying bilayer structure. An extra light membrane fraction which results from membrane preparations utilizing a French pressure cell for spheroplast disruption has been characterized and shown to be similar to inner membrane. The purified membranes produce small angle X-ray diffraction patterns which are much different from those of lipid dispersions and the differences are attributable to the high protein content of the intact membranes. While the small angle X-ray region may be useful for characterizing the membrane preparations, the paucity of detail in the diffraction pattern suggest that it will be of little value in describing the complex underlying membrane structure.
已获得来自大肠杆菌内膜（或胞质膜）和外膜纯化形式的小角X射线数据，且这些数据在质量上似乎相似。内膜的圆平均X射线图谱中明显存在短暂变化。这种结果可能是由于外周膜蛋白的丢失或变性所致。外膜的一些部分干燥形式呈部分有序状态，并产生支持潜在双层结构的衍射图样。利用法国压榨器破碎原生质球进行膜制备时产生的一种额外的轻膜组分已得到表征，且显示其与内膜相似。纯化后的膜产生的小角X射线衍射图样与脂质分散体的衍射图样有很大不同，这些差异归因于完整膜中蛋白质含量高。虽然小角X射线区域可能有助于表征膜制备物，但衍射图样中细节的匮乏表明，它在描述复杂的潜在膜结构方面价值不大。
