Zhao Yuanyuan, Le Wenjing, Genco Caroline A, Rice Peter A, Su Xiaohong
Sexually Transmitted Disease Clinic, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, People's Republic of China.
Department of Immunology, Tufts University School of Medicine, Boston, MA, USA.
Infect Drug Resist. 2023 Jun 23;16:4053-4064. doi: 10.2147/IDR.S408896. eCollection 2023.
Since the first Chinese report of the ceftriaxone-resistant FC428 clone in 2016, additional FC428-like, 60.001 isolates have been identified in China.
To document the rise in 60.001 isolates in Nanjing, China, and characterize their molecular and epidemiological features.
minimum inhibitory concentrations (MICs, mg/L) for ceftriaxone, cefixime, penicillin, tetracycline, ciprofloxacin, azithromycin, spectinomycin, gentamicin and zoliflodacin were determined by agar dilution. MICs for ertapenem were measured by E-test. N. antimicrobial sequence typing (NG-STAR) of seven loci ( and ) was analyzed together with multiantigen sequence typing (NG-MAST) and multilocus sequence typing (MLST). Phylogenetic analysis was also performed using whole genomic sequencing (WGS).
Fourteen FC428-related 60.001 infections were identified out of 677 infections from 2017 to 2020, in Nanjing, representing an incremental yearly rise in the percentage of the city's isolates that were FC428-related. Seven FC428-related N. infections were acquired in Nanjing, proper; four others in eastern Chinese cities and three from unknown locations. All FC428-related isolates were resistant to ceftriaxone, cefixime, ciprofloxacin, tetracycline and penicillin but susceptible to spectinomycin, gentamicin, ertapenem and zoliflodacin; three strains were resistant to azithromycin. 60.001 isolates displayed closely related MLST types and NG-STAR types but relatively distant NG-MAST types. WGS showed a phylogenetic analysis that intermingled with other international isolates.
60.001 isolates emerged in Nanjing, China, beginning in 2017, and have continued to rise.
自2016年中国首次报告对头孢曲松耐药的FC428克隆以来，中国已鉴定出更多类似FC428的60.001分离株。
记录中国南京60.001分离株的增加情况，并描述其分子和流行病学特征。
采用琼脂稀释法测定头孢曲松、头孢克肟、青霉素、四环素、环丙沙星、阿奇霉素、大观霉素、庆大霉素和佐利福达辛 的最低抑菌浓度（MIC，mg/L）。采用E-test法测定厄他培南的MIC。对7个位点（和）进行淋病奈瑟菌抗菌序列分型（NG-STAR），并与多抗原序列分型（NG-MAST）和多位点序列分型（MLST）一起分析。还使用全基因组测序（WGS）进行系统发育分析。
在南京2017年至2020年的677例感染中，鉴定出14例与FC428相关的60.001感染，这表明该市与FC428相关的分离株百分比逐年上升。7例与FC428相关的淋病奈瑟菌感染在南京市区获得；另外4例在华东城市，3例来源不明。所有与FC428相关的分离株均对头孢曲松、头孢克肟、环丙沙星、四环素和青霉素耐药，但对大观霉素、庆大霉素、厄他培南和佐利福达辛敏感；3株对阿奇霉素耐药。60.001分离株显示出密切相关的MLST类型和NG-STAR类型，但NG-MAST类型相对较远。WGS显示系统发育分析结果与其他国际分离株混合在一起。
60.001分离株于2017年开始在中国南京出现，并持续增加。
