紫草素通过Ras1-cAMP-Efg1信号通路抑制生物膜形成。
Shikonin Inhibits Biofilms via the Ras1-cAMP-Efg1 Signalling Pathway.
作者信息
Pang Chong, Chen Jianshuang, Yang Lan, Yang Yang, Qi Haihua, Li Ran, Cao Yingying, Miao Hao
机构信息
School of Basic Medicine, Chengde Medical University, Chengde, Hebei, People's Republic of China.
Hebei Key Laboratory of Nerve Injury and Repair, Chengde, Hebei, People's Republic of China.
出版信息
Int J Gen Med. 2023 Jun 23;16:2653-2662. doi: 10.2147/IJGM.S417327. eCollection 2023.
OBJECTIVE
To investigate the influence of shikonin (SK) on the formation of biofilms and discuss the possible mechanism.
METHODS
The inhibition of the formation of biofilms by SK was observed by scanning electron microscopy. A silicone film method and a water-hydrocarbon two-phase assay were performed to investigate the effects of SK on cell adhesion. Real-time reverse-transcription polymerase chain reaction was used to analyse the expression of genes related to cell adhesion and Ras1-cyclic adenosine monophosphate (cAMP) - enhanced filamentous growth protein 1 (Efg1) signalling pathway. Finally, the level of cAMP in was detected and exogenous cAMP rescue experiment was conducted.
RESULTS
The results showed that SK could destroy the typical three-dimensional structure of the biofilms, inhibit cell surface hydrophobicity and cell adhesion, downregulate the expression of Ras1-cAMP-Efg1 signalling pathway-related genes ( and ) and effectively reduce the production of key messenger cAMP in the Ras1-cAMP-Efg1 pathway. Meanwhile, exogenous cAMP reversed the inhibitory effect of SK on biofilms formation.
CONCLUSION
Our results suggest that SK exhibits potential anti- biofilms effects related to the inhibition of Ras1-cAMP-Efg1 pathway.
目的
研究紫草素（SK）对生物膜形成的影响并探讨其可能机制。
方法
通过扫描电子显微镜观察SK对生物膜形成的抑制作用。采用硅膜法和水 - 烃两相分析法研究SK对细胞黏附的影响。运用实时逆转录聚合酶链反应分析与细胞黏附及Ras1 - 环磷酸腺苷（cAMP） - 增强丝状生长蛋白1（Efg1）信号通路相关基因的表达。最后，检测细胞内cAMP水平并进行外源性cAMP拯救实验。
结果
结果显示，SK可破坏生物膜典型的三维结构，抑制细胞表面疏水性和细胞黏附，下调Ras1 - cAMP - Efg1信号通路相关基因（ 和 ）的表达，并有效降低Ras1 - cAMP - Efg1通路中关键信使cAMP的产生。同时，外源性cAMP逆转了SK对生物膜形成的抑制作用。
结论
我们的结果表明，SK通过抑制Ras1 - cAMP - Efg1通路表现出潜在的抗生物膜作用。
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