Centre for Urban Environmental Remediation, Beijing University of Civil Engineering and Architecture, Beijing, 100044, China.
Centre for Urban Environmental Remediation, Beijing University of Civil Engineering and Architecture, Beijing, 100044, China; Collaborative Innovation Center of Energy Conservation & Emission Reduction and Sustainable Urban-Rural Development in Beijing, Beijing University of Civil Engineering and Architecture, Beijing, 100044, China; Key Laboratory of Urban Stormwater System & Water Environment (Ministry of Education), Beijing University of Civil Engineering and Architecture, Beijing, 100044, China.
J Environ Manage. 2023 Oct 15;344:118460. doi: 10.1016/j.jenvman.2023.118460. Epub 2023 Jun 27.
The effective retention of anaerobic ammonia oxidizing (anammox) bacteria and its high sensitivity to toxic substances and oxygen posed a major challenge to the application of partial nitrification combined with anammox (PN/A) in mature landfill leachate treatment, although it is a promising and efficient nitrogen removal process. In this study, a single-stage PN/A process based on expanded granular sludge bed was proposed to treat the mature landfill leachate. During the last phase, when the NH+ 4-N concentration of mature landfill leachate in influent was 1150.0 mg/L, the nitrogen removal efficiency (NRE) was 83.64% with 1.07 kg N/(m·d) nitrogen removal rate (NRR). The activity of anammox bacteria (AnAOB) and ammonia oxidizing bacteria (AOB) was 9.21 ± 0.22 mg N/(gVSS·h) and 14.34 ± 0.65 mg N/(gVSS·h), respectively. The bacteria produced a high amount of tightly bound extracellular polymeric substance (TB-EPS) i.e., 4071.79 mg/(g·VSS). This helped to create granular sludge and provided favorable spatial conditions for the distribution of functional bacteria that were adapted to different environments. Due to the efficient retention of functional bacteria by the granular sludge, the relative abundance of Ca.Brocadia and Ca.Kuneneia was 1.71% and 0.31%, respectively. Redundancy analysis (RDA) and microbial correlation network diagram showed that the relative abundance of Ca. Kuenenia, Nitrosomonas and Truepera had a stronger positive correlation with the increase of the proportion of mature landfill leachate added to the influent. Overall, the PN/A process based on granular sludge provides an effective method for autotrophic biological nitrogen removal from mature landfill leachate.
尽管部分硝化联合厌氧氨氧化（PN/A）是一种很有前途且高效的脱氮工艺，但厌氧氨氧化菌（Anammox）的有效保留及其对有毒物质和氧气的高敏感性对其在成熟垃圾渗滤液处理中的应用构成了重大挑战。在本研究中，提出了一种基于膨胀颗粒污泥床的单级 PN/A 工艺来处理成熟垃圾渗滤液。在最后阶段，当进水的成熟垃圾渗滤液中 NH+ 4-N 浓度为 1150.0mg/L 时，氮去除效率（NRE）为 83.64%，氮去除速率（NRR）为 1.07kgN/（m·d）。厌氧氨氧化菌（AnAOB）和氨氧化菌（AOB）的活性分别为 9.21±0.22mgN/（gVSS·h）和 14.34±0.65mgN/（gVSS·h）。细菌产生了大量紧密结合的胞外聚合物（TB-EPS），即 4071.79mg/（g·VSS）。这有助于形成颗粒污泥，并为适应不同环境的功能细菌的分布提供了有利的空间条件。由于颗粒污泥对功能细菌的有效保留，Ca.Brocadia 和 Ca.Kuneneia 的相对丰度分别为 1.71%和 0.31%。冗余分析（RDA）和微生物相关网络图显示，Ca. Kuenenia、Nitrosomonas 和 Truepera 的相对丰度与进水添加的成熟垃圾渗滤液比例的增加呈更强的正相关。总体而言，基于颗粒污泥的 PN/A 工艺为从成熟垃圾渗滤液中进行自养生物脱氮提供了一种有效方法。
