State Key Laboratory of Food Science and Technology, No. 235 Nanjing East Road, Nanchang, Jiangxi 330047, P. R. China.
School of Food Science and Technology, Nanchang University, No. 235 Nanjing East Road, Nanchang, Jiangxi 330047, P. R. China.
J Agric Food Chem. 2023 Jul 12;71(27):10361-10374. doi: 10.1021/acs.jafc.3c03149. Epub 2023 Jun 30.
The major characteristics of obesity are abnormal lipid metabolism, chronic inflammation, and imbalanced gut microbiota. It has been reported that lactic acid bacteria (LAB) possess potential for alleviating obesity, considering which the strain-specific functions and diverse mechanisms and the roles and mechanisms of various LAB are worthy of investigation. This study aimed to validate and investigate the alleviating effects and underlying mechanisms of three LAB strains, NCUH001046 (LP), NCUH064003, and NCUH003068 (LF), in high-fat-diet-induced obese mice. The findings demonstrated that the three strains, particularly LP, suppressed body weight gain and fat deposition; ameliorated lipid disorders, liver and adipocyte morphology, and chronic low-grade inflammation; and reduced lipid synthesis via activating the adenosine 5'-monophosphate-activated protein kinase (AMPK) signaling pathway. In addition, LP and LF decreased the enrichment of bacteria positively correlated with obesity, like , , and , but facilitated the growth of beneficial bacteria negatively correlated with obesity, like , , and , along with increasing the short-chain fatty acid levels. It is deduced that the underlying alleviating mechanism of LP was to modulate the hepatic AMPK signaling pathway and gut microbiota by the microbiome-fat-liver axis to alleviate obesity development. In conclusion, as a diet supplement, LP has promising potential in obesity prevention and treatment.
肥胖的主要特征是脂质代谢异常、慢性炎症和肠道菌群失衡。据报道，乳酸菌（LAB）具有缓解肥胖的潜力，因此值得研究其菌株特异性功能、多种机制以及不同 LAB 的作用和机制。本研究旨在验证和研究三种 LAB 菌株，即 NCUH001046（LP）、NCUH064003 和 NCUH003068（LF），在高脂饮食诱导的肥胖小鼠中的缓解作用及其潜在机制。研究结果表明，这三种菌株，特别是 LP，可抑制体重增加和脂肪沉积；改善脂代谢紊乱、肝脏和脂肪细胞形态以及慢性低度炎症；并通过激活腺苷 5'-单磷酸激活蛋白激酶（AMPK）信号通路来减少脂质合成。此外，LP 和 LF 减少了与肥胖呈正相关的细菌的丰度，如 、 、 和 ，但促进了与肥胖呈负相关的有益细菌的生长，如 、 、 和 ，同时增加了短链脂肪酸水平。可以推断，LP 的潜在缓解机制是通过微生物群-脂肪-肝脏轴来调节肝脏 AMPK 信号通路和肠道菌群，从而缓解肥胖的发展。总之，作为一种饮食补充剂，LP 在肥胖的预防和治疗方面具有广阔的应用前景。
