Laboratório de Química dos Produtos Naturais, Universidade do Estado do Pará, Belém, 66087-662, Brazil; Laboratório de Morfofisiologia Aplicada a Saúde, Departamento de Morfologia e Ciências Fisiológicas, Universidade do Estado do Pará, Belém, 66087-662, Brazil.
Laboratório de Morfofisiologia Aplicada a Saúde, Departamento de Morfologia e Ciências Fisiológicas, Universidade do Estado do Pará, Belém, 66087-662, Brazil.
J Ethnopharmacol. 2023 Dec 5;317:116859. doi: 10.1016/j.jep.2023.116859. Epub 2023 Jun 28.
The Eugenia uniflora leaf infusion is widely used in folk medicine to treat gastroenteritis, fever, hypertension, inflammatory and diuretic diseases.
This work evaluated the acute oral toxic, antinociceptive, and anti-inflammatory activities of the curzerene chemotype of Eugenia uniflora essential oil (EuEO).
EuEO was obtained by hydrodistillation and analyzed by GC and GC-MS. The antinociceptive action in mice was evaluated for the peripheral and central analgesic activity using abdominal contortion and hot plate tests (50, 100, and 200 mg/kg); xylene-induced ear swelling was carried out for the nociception test, and carrageenan-induced cell migration test. Spontaneous locomotor activity was assessed in the open field test to rule out any nonspecific sedative or muscle relaxant effects of EuEO.
The EuEO displayed a yield of 2.6 ± 0.7%. The major compounds classes were oxygenated sesquiterpenoids (57.3 ± 0.2%), followed by sesquiterpene hydrocarbons (16.4 ± 2.6). The chemical constituents with the highest concentrations were curzerene (33.4 ± 8.5%), caryophyllene oxide (7.6 ± 2.8%), β-elemene (6.5 ± 1.8%), and E-caryophyllene (4.1 ± 0.3%). Oral treatment with EuEO, at doses of 50, 300, and 2000 mg/kg, did not change the behavior patterns or mortality of the animals. EuEO (300 mg/kg) did not cause a reduction in the number of crossings in the open field compared to the vehicle group. The aspartate aminotransferase (AST) level was higher in EuEO-treated groups (50 and 2000 mg/kg) when compared to the control group (p < 0.05). EuEO, at doses of 50, 100, and 200 mg/kg, reduced the number of abdominal writhings by 61.66%, 38.33%, and 33.33%. EuEO did not show increased hot plate test time latency in any of the intervals analyzed. At 200 mg/kg, EuEO decreased paw licking time, with inhibition of 63.43%. In formalin-induced acute pain, EuEO decreased paw licking time at doses of 50, 100, and 200 mg/kg in the first phase, with inhibition of 30.54%, 55.02%, and 80.87%. The groups treated with EuEO at doses of 50, 100, and 200 mg/kg showed ear edema reduction of 50.26%, 55.17%, and 51.31%, respectively. Moreover, EuEO inhibited leukocyte recruitment only at a dose of 200 mg/kg. The inhibitory values of leukocyte recruitment after 4 h of carrageenan application were 4.86%, 4.93%, and 47.25% for 50, 100, and 200 mg/kg of essential oil, respectively.
The EuEO, curzerene chemotype, has significant antinociceptive and anti-inflammatory activities and low acute oral toxicity. This work confirms the antinociceptive and anti-inflammatory of this species as the traditional use.
Eugenia uniflora 的叶子浸剂在民间医学中被广泛用于治疗胃肠炎、发热、高血压、炎症和利尿疾病。
本研究评估 Eugenia uniflora 精油（EuEO）中 curzerene 化学型的急性口服毒性、镇痛和抗炎活性。
EuEO 通过水蒸馏获得，并通过 GC 和 GC-MS 进行分析。使用腹部扭曲和热板试验（50、100 和 200mg/kg）评估小鼠的外周和中枢镇痛作用；进行二甲苯诱导的耳肿胀以进行痛觉测试，并进行角叉菜胶诱导的细胞迁移试验。在旷场试验中评估自发运动活动，以排除 EuEO 的任何非特异性镇静或肌肉松弛作用。
EuEO 的产率为 2.6±0.7%。主要化合物类别为含氧倍半萜（57.3±0.2%），其次是倍半萜烃（16.4±2.6%）。浓度最高的化学成分是 curzerene（33.4±8.5%）、石竹烯氧化物（7.6±2.8%）、β-榄香烯（6.5±1.8%）和 E-石竹烯（4.1±0.3%）。口服给予 50、300 和 2000mg/kg 的 EuEO 剂量不会改变动物的行为模式或死亡率。与载体组相比，EuEO（300mg/kg）在开放场中没有减少穿越次数。与对照组相比，EuEO 治疗组（50 和 2000mg/kg）的天门冬氨酸氨基转移酶（AST）水平更高（p<0.05）。EuEO 在 50、100 和 200mg/kg 剂量下，使腹部扭曲次数减少了 61.66%、38.33%和 33.33%。EuEO 在任何分析的间隔时间内均未增加热板试验时间延迟。在 200mg/kg 时，EuEO 减少了爪舔时间，抑制率为 63.43%。在福尔马林诱导的急性疼痛中，EuEO 在 50、100 和 200mg/kg 剂量下减少了第一阶段的爪舔时间，抑制率分别为 30.54%、55.02%和 80.87%。EuEO 在 50、100 和 200mg/kg 剂量下的耳肿胀减少分别为 50.26%、55.17%和 51.31%。此外，EuEO 仅在 200mg/kg 剂量下抑制白细胞募集。角叉菜胶应用 4 小时后，白细胞募集的抑制值分别为 50、100 和 200mg/kg 的精油的 4.86%、4.93%和 47.25%。
EuEO，curzerene 化学型具有显著的镇痛和抗炎活性和低急性口服毒性。这项工作证实了该物种的镇痛和抗炎作用，如其传统用途。
