诱导多能干细胞系 IITGi001-A 的建立及特性鉴定，该细胞系来源于成人原代皮肤成纤维细胞。
Generation and characterization of induced pluripotent stem cell line IITGi001-A derived from adult human primary dermal fibroblasts.
机构信息
Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
Department of Hematology, Christian Medical College, Vellore, India.
出版信息
Stem Cell Res. 2023 Sep;71:103159. doi: 10.1016/j.scr.2023.103159. Epub 2023 Jun 28.
Adult human primary dermal fibroblasts [ATCC (PCS-201-012)] were reprogrammed by transfection of oriP/EBNA-1 based episomal plasmids expressing OCT3/4, SOX2, KLF4, L-MYC, LIN28 and a p53 shRNA (Okita et al., 2011) to give rise to induced pluripotent stem cells (iPSCs). These iPSCs expressed core pluripotency markers, maintained normal karyotype, and showed tri-lineage differentiation potential. Further, the absence of episomal plasmid integration in this iPSC line was confirmed by genomic PCR. In addition, DNA fingerprinting of fibroblast and iPSC DNA by microsatellite analysis confirmed the genetic identity of this cell line. This iPSC line was shown to be free from mycoplasma contamination.
成体人原代真皮成纤维细胞 [ATCC（PCS-201-012）] 通过转染表达 OCT3/4、SOX2、KLF4、L-MYC、LIN28 和 p53 shRNA 的 oriP/EBNA-1 基于附加体的质粒进行重编程，从而产生诱导多能干细胞 (iPSC)。这些 iPSC 表达核心多能性标志物，保持正常核型，并显示三系分化潜能。此外，通过基因组 PCR 证实该 iPSC 系中不存在附加体质粒整合。此外，通过微卫星分析对成纤维细胞和 iPSC DNA 的 DNA 指纹分析证实了该细胞系的遗传同一性。该 iPSC 系被证明无支原体污染。
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