将一名 48 岁健康男性供者的人诱导多能干细胞系进行重编程。
Reprogramming of a human induced pluripotent stem cell line from one 48-year-old healthy male donor.
机构信息
Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China.
Center of Prenatal Diagnosis, Shijiazhuang Obstetrics and Gynecology Hospital, Shijiazhuang, Hebei 050011, China.
出版信息
Stem Cell Res. 2021 May;53:102339. doi: 10.1016/j.scr.2021.102339. Epub 2021 Apr 20.
In this study, skin biopsy was collected from a healthy 48-year old male donor with informed consent, and the fibroblasts were isolated from the dermal explant cultures. Here, a human induced pluripotent stem cell (iPSC) line was derived from the fibroblasts using the reprogramming four Yamanaka factors (Oct3/4, Sox2, Klf4, c-Myc). The generated iPSCs were integration-free, displayed the normal karyotype, expressed pluripotency markers and demonstrated trilineage differentiation potential in vitro. This iPSC model will be useful for investigating physiological processes, drug validation as well as a control in pathological mechanistic studies.
在这项研究中，我们从一名 48 岁的健康男性供体身上采集了皮肤活检样本，并获得了知情同意，然后从皮肤外植体培养物中分离出成纤维细胞。在此基础上，我们使用重编程的四个 Yamanaka 因子（Oct3/4、Sox2、Klf4 和 c-Myc）从成纤维细胞中成功诱导出了一个人类诱导多能干细胞（iPSC）系。该 iPSC 系无整合，具有正常核型，表达多能性标志物，并在体外具有三系分化潜能。该 iPSC 模型将有助于研究生理过程、药物验证以及在病理性机制研究中作为对照。
Zotero 插件