School of Public Health, Zunyi Medical University, No. 6, Xuefu West Road, Zunyi 563000, Guizhou, China.
School of Public Health, Zunyi Medical University, No. 6, Xuefu West Road, Zunyi 563000, Guizhou, China.
Exp Gerontol. 2023 Aug;179:112249. doi: 10.1016/j.exger.2023.112249. Epub 2023 Jul 3.
Previous studies have indicated that serum sex hormone-binding globulin (SHBG) levels increase with age; however, the causes remain unknown. The present study aimed to clarify whether the increase in SHBG levels is attributable to aging-related increases in SHBG synthesis.
We examined and evaluated the association of serum SHBG levels with synthesis-related factors in men aged 18-80 years. Additionally, we examined the serum and liver levels of SHBG, hepatic nuclear factor 4α (HNF-4α), and peroxisome proliferator-activated receptor γ (PPAR-γ) in young, middle-aged, and old Sprague-Dawley rats.
The study included 209 men in the young group (median age, 33 ± 10 years), 174 men in the middle-aged group (median age, 53 ± 8 years), and 98 men in the elderly group (median age, 71 ± 8 years). Serum SHBG levels increased with age (P < 0.05), whereas HNF-4α and PPAR-γ levels decreased with age (both P < 0.05). Compared with the findings in the young group, the average decline in HNF-4α levels was 2.61 % and 18.46 % in the middle-aged and elderly groups, respectively; the average decreases in PPAR-γ levels in these groups were 12.86 % and 20.76 %, respectively. The results in rats illustrated that liver SHBG and HNF-4α levels increased with age, whereas PPAR-γ and chicken ovalbumin upstream promoter-transcription factor (COUP-TF) levels decreased with age (all P < 0.05). Serum SHBG levels increased in rats with age, whereas HNF-4α and PPAR-γ levels decreased with age (all P < 0.05).
The aging-related increased liver levels of the SHBG synthesis promoter HNF-4α and decreased levels of the SHBG inhibitory factors PPAR-γ and COUP-TF suggest that the aging-related increases in SHBG levels are associated with increased SHBG synthesis.
先前的研究表明，血清性激素结合球蛋白（SHBG）水平随年龄增长而增加；然而，其原因尚不清楚。本研究旨在阐明 SHBG 水平的升高是否归因于与衰老相关的 SHBG 合成增加。
我们检查并评估了 18-80 岁男性的血清 SHBG 水平与合成相关因素的关系。此外，我们检查了年轻、中年和老年 Sprague-Dawley 大鼠的血清和肝脏 SHBG、核因子 4α（HNF-4α）和过氧化物酶体增殖物激活受体γ（PPAR-γ）水平。
该研究纳入了 209 名年轻组（中位年龄 33±10 岁）、174 名中年组（中位年龄 53±8 岁）和 98 名老年组（中位年龄 71±8 岁）男性。血清 SHBG 水平随年龄增长而升高（P<0.05），而 HNF-4α 和 PPAR-γ 水平随年龄增长而降低（均 P<0.05）。与年轻组相比，HNF-4α 水平在中年和老年组的平均下降率分别为 2.61%和 18.46%；PPAR-γ 水平在这两组中的平均下降率分别为 12.86%和 20.76%。大鼠研究结果表明，肝脏 SHBG 和 HNF-4α 水平随年龄增长而增加，而 PPAR-γ 和鸡卵清蛋白上游启动子转录因子（COUP-TF）水平随年龄增长而降低（均 P<0.05）。随着大鼠年龄的增长，血清 SHBG 水平增加，而 HNF-4α 和 PPAR-γ 水平随年龄增长而降低（均 P<0.05）。
与衰老相关的 SHBG 合成启动子 HNF-4α 的肝脏水平升高以及 SHBG 抑制因子 PPAR-γ 和 COUP-TF 的水平降低表明，与衰老相关的 SHBG 水平升高与 SHBG 合成增加有关。
