CRISPR/Cas9 基因编辑技术:一种用于改善作物品质的精确高效工具。

CRISPR/Cas9 gene editing technology: a precise and efficient tool for crop quality improvement.

机构信息

College of Biological and Chemical Engineering, Qilu Institute of Technology, Jinan, 250200, Shandong, People's Republic of China.

College of Life Sciences, Linyi University, Linyi, 276000, Shandong, People's Republic of China.

出版信息

Planta. 2023 Jul 3;258(2):36. doi: 10.1007/s00425-023-04187-z.

Abstract

This review provides a direction for crop quality improvement and ideas for further research on the application of CRISPR/Cas9 gene editing technology for crop improvement. Various important crops, such as wheat, rice, soybean and tomato, are among the main sources of food and energy for humans. Breeders have long attempted to improve crop yield and quality through traditional breeding methods such as crossbreeding. However, crop breeding progress has been slow due to the limitations of traditional breeding methods. In recent years, clustered regularly spaced short palindromic repeat (CRISPR)/Cas9 gene editing technology has been continuously developed. And with the refinement of crop genome data, CRISPR/Cas9 technology has enabled significant breakthroughs in editing specific genes of crops due to its accuracy and efficiency. Precise editing of certain key genes in crops by means of CRISPR/Cas9 technology has improved crop quality and yield and has become a popular strategy for many breeders to focus on and adopt. In this paper, the present status and achievements of CRISPR/Cas9 gene technology as applied to the improvement of quality in several crops are reviewed. In addition, the shortcomings, challenges and development prospects of CRISPR/Cas9 gene editing technology are discussed.

摘要

本文综述了 CRISPR/Cas9 基因编辑技术在几种作物品质改良中的应用现状和成果,为作物品质改良提供了方向和思路,同时也为该技术在作物改良中的进一步研究提供了参考。小麦、水稻、大豆和番茄等各种重要作物是人类食物和能源的主要来源。长期以来,育种家们一直试图通过杂交等传统的育种方法来提高作物的产量和品质。然而,由于传统育种方法的局限性,作物的育种进展一直较为缓慢。近年来,CRISPR/Cas9 基因编辑技术不断发展,并随着作物基因组数据的精细化,CRISPR/Cas9 技术因其准确性和高效性,使得对作物特定基因的编辑取得了显著突破。通过 CRISPR/Cas9 技术对作物中某些关键基因进行精确编辑,提高了作物的品质和产量,已成为许多育种家关注和采用的热门策略。本文综述了 CRISPR/Cas9 基因编辑技术在几种作物品质改良中的应用现状和成果,此外,还讨论了 CRISPR/Cas9 基因编辑技术的缺点、挑战和发展前景。

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