Sastre Domínguez J, Sastre Castillo A
Allergol Immunopathol (Madr). 1986 May-Jun;14(3):221-8.
We have evaluated the usefulness of HBDT as an in vitro method for the diagnosis of drug allergy. Two hundred and thirty six patients with suspected drug sensitization to penicillin, streptomycin, sulfamides, pyrazolones and A.S.A. were analyzed. Seventy-nine of them were allergic; in 43 cases it was confirmed by in vivo methods. Other patients were diagnosed by clinical history only if they had more than two reactions to the same drug. In order to be included in this group patients with reactions to pyrazolones and A.S.A. had to have tolerated other NSAI, therefore these patients were allergic to one compound only. All patients were considered non-allergic were determined by a negative provocation test. In the group of allergic patients we obtained 63 (79%) positive degranulations and 16 (21%) negative. One hundred and thirty two (84%) negative degranulations and 25 (16%) positive were obtained in the group of non-allergic patients. Once having analyzed 10 statistical parameters with each drug, the HBOT appears to be a useful method for these drugs except for streptomycin. In 16 (80%) out of 20 aspirin sensitive asthmatic patients we found that their basophils were degranulated. In 7 patients with urticaria and/or angioedema by A.S.A. and other NSAI the degranulation was negative, confirming the absence of the involvement of basophils in this reactions.
我们评估了嗜碱性粒细胞脱颗粒试验（HBDT）作为诊断药物过敏的体外方法的有效性。分析了236例疑似对青霉素、链霉素、磺胺类、吡唑啉酮和阿司匹林过敏的患者。其中79例过敏；43例通过体内方法得到证实。其他患者仅在对同一药物有两种以上反应时才根据临床病史进行诊断。为了纳入该组，对吡唑啉酮和阿司匹林有反应的患者必须耐受其他非甾体抗炎药（NSAI），因此这些患者仅对一种化合物过敏。所有被认为非过敏的患者均通过阴性激发试验确定。在过敏患者组中，我们获得了63例（79%）阳性脱颗粒和16例（21%）阴性脱颗粒。在非过敏患者组中，获得了132例（84%）阴性脱颗粒和25例（16%）阳性脱颗粒。在用每种药物分析了10个统计参数后，除链霉素外，嗜碱性粒细胞脱颗粒试验（HBOT）似乎是检测这些药物过敏的一种有用方法。在20例阿司匹林敏感的哮喘患者中，有16例（80%）发现其嗜碱性粒细胞脱颗粒。在7例因阿司匹林和其他非甾体抗炎药出现荨麻疹和/或血管性水肿的患者中，脱颗粒试验为阴性，证实嗜碱性粒细胞未参与此反应。
