College of Ecology and Resource Engineering, Wuyi University, Wuyishan, China.
College of Tea and Food, Wuyi University, Wuyishan, China.
J Hazard Mater. 2023 Sep 15;458:131957. doi: 10.1016/j.jhazmat.2023.131957. Epub 2023 Jun 28.
Accumulation of phenolic acids, such as p-hydroxybenzoic acid (PHBA), 3,4 dihydroxybenzoic acid (PA), and cinnamic acid (CA) causes a decline in tea plantation soil quality. Bacterial strains that can balance phenolic acid autotoxicity (PAA) in tea tree rhizosphere soil are used to improve tea plantation soil. In this study, the effects of Pseudomonas fluorescens ZL22 on soil restoration and PAA regulation in tea plantations were investigated. ZL22 carries a complete pathway for degrading PHBA and PA to acetyl coenzyme A. ZL22 can colonise and reduce PHBA by 96% and PA by 98% in tea rhizosphere soil within 30 days. The cooccurrence of ZL22 and low CA levels further promotes lettuce seed growth and substantially increases tea production. ZL22 effectively regulates PAA to a safe level in rhizospheric soil, alleviating the inhibition of microbiota by PAA, increases the abundance of genera associated with soil N, C, and S cycling, and creates optimum pH (approximately 4.2) and organic carbon (approximately 25 g/kg), and available N (approximately 62 mg/kg) contents for secondary metabolite accumulation in tea leaves. The application of P. fluorescens ZL22 controls PAA, which synergistically improves plant growth and soil nutrition, thereby promoting tea production and quality.
酚酸（如对羟基苯甲酸（PHBA）、3,4-二羟基苯甲酸（PA）和肉桂酸（CA））的积累会导致茶园土壤质量下降。能够平衡茶树根际土壤中酚酸自毒性（PAA）的细菌菌株被用于改良茶园土壤。本研究调查了荧光假单胞菌 ZL22 对茶园土壤修复和 PAA 调节的影响。ZL22 携带一条完整的代谢途径，可将 PHBA 和 PA 降解为乙酰辅酶 A。ZL22 可在 30 天内在茶根际土壤中定殖并将 PHBA 降低 96%，将 PA 降低 98%。ZL22 的存在和低 CA 水平进一步促进生菜种子生长，并显著提高茶叶产量。ZL22 可有效地将 PAA 调节到根际土壤的安全水平，缓解 PAA 对微生物群的抑制作用，增加与土壤 N、C 和 S 循环相关的属的丰度，并创造最佳 pH 值（约 4.2）和有机碳（约 25 g/kg）以及有效氮（约 62 mg/kg）含量，有利于茶叶中次生代谢物的积累。荧光假单胞菌 ZL22 的应用控制了 PAA，从而协同促进了植物生长和土壤养分，从而提高了茶叶的产量和质量。
