Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture, Beijing, China.
College of Landscape Architecture, Beijing University of Agriculture, Beijing, China.
J Appl Microbiol. 2021 Jul;131(1):208-220. doi: 10.1111/jam.14956. Epub 2020 Dec 15.
To isolate, identify and characterize phenolic acid-degrading bacteria and reduce plant growth inhibition caused by phenolic acids.
A total of 11 bacterial isolates with high phthalic acid (PA)-degrading ability were obtained using mineral salt medium (MSM) medium containing PA as sole carbon source. These isolates were identified as Arthrobacter globiformis, Pseudomonas putida and Pseudomonas hunanensis by sequence analyses of the 16S rRNA gene. Among them, five Pseudomonas strains could also effectively degrade ferulic acid (FA), p-hydroxybenzoic acid (PHBA) and syringic acid (SA) in MSM solution. P. putida strain 7 and P. hunanensis strain 10 showed highly efficient degradation of PA, SA, FA and PHBA, and could reduce their inhibition of lily, watermelon, poplar and strawberry seedling growth in soils respectively. These two strains could promote plant growth in soil with phenolic acids.
In this study, bacterial strains with highly efficient phenolic acid-degrading abilities could not only effectively reduce the autotoxicity of phenolic acids on plants but also were able to promote plant growth in soil with phenolic acids.
In this study, Pseudomonas can promote plant growth while degrading phenolic acids. Our results provide new choices for the biological removal of autotoxins.
分离、鉴定和表征能够降解酚酸的细菌，并减轻酚酸对植物生长的抑制作用。
采用以邻苯二甲酸（PA）为唯一碳源的无机盐培养基（MSM），获得了 11 株具有高邻苯二甲酸（PA）降解能力的细菌分离株。通过 16S rRNA 基因序列分析，这些分离株被鉴定为球形节杆菌、假单胞菌和湖南假单胞菌。其中，5 株假单胞菌菌株也能有效地在 MSM 溶液中降解阿魏酸（FA）、对羟基苯甲酸（PHBA）和丁香酸（SA）。假单胞菌菌株 7 和湖南假单胞菌菌株 10 对 PA、SA、FA 和 PHBA 的降解效率很高，分别能减轻它们对百合、西瓜、杨树和草莓幼苗在土壤中生长的抑制作用。这两株菌能促进土壤中含有酚酸的植物生长。
在本研究中，具有高效酚酸降解能力的细菌菌株不仅能有效降低酚酸对植物的自毒性，还能促进土壤中含有酚酸的植物生长。
在本研究中，假单胞菌既能促进植物生长，又能降解酚酸。我们的研究结果为生物去除自毒素提供了新的选择。
