WNT 家族成员和 RUNX2 基因变异对牙齿和骨骼成熟度的影响：一项横断面研究。

Impact of genetic variations in the WNT family members and RUNX2 on dental and skeletal maturation: a cross-sectional study.

机构信息

Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.

School of Dentistry, Tuiuti University of Paraná, Paraná, Brazil.

出版信息

Head Face Med. 2023 Jul 3;19(1):26. doi: 10.1186/s13005-023-00372-3.

DOI:10.1186/s13005-023-00372-3

PMID:37400934

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10316614/

Abstract

BACKGROUND

This study evaluated if genetic variations in the WNT family members and RUNX2 are associated with craniofacial maturation, investigating dental and skeletal maturity in children and teenagers.

METHODS

Radiographs from pre-orthodontic treatment of Brazilian patients (7 to 17 years-old) were used to assess dental (panoramic radiographs) and skeletal maturity (cephalometric radiographs). The chronological age (CA) was calculated based on the date of birth and the time the radiographs were performed. For the dental maturity analysis, the Demirjian (1973) method was used and a delta [dental age - chronological age (DA-CA)] was calculated. For the skeletal maturity analysis, the Baccetti et al. (2005) method was used and the patients were classified as "delayed skeletal maturation", "advanced skeletal maturation" or "normal skeletal maturation". DNA isolated from buccal cells was used for genotyping of two genetic variations in WNT family genes: rs708111 (G > A) in WNT3A and rs1533767 (G > A) in WNT11; and two genetic variations in RUNX2: rs1200425 (G > A) and rs59983488 (G > T). A statistical analysis was performed and values of p < 0.05 indicated a significant difference.

RESULTS

There were no associations between dental maturity and genotypes (p > 0.05). In the skeletal maturity analysis, the allele A in the rs708111 (WNT3A) was statistically more frequent in patients with delayed skeletal maturation (Prevalence Ratio = 1.6; 95% Confidence Interval = 1.00 to 2.54; p-value = 0.042).

CONCLUSIONS

The rs708111 in the WNT3A gene impacts on skeletal maturation.

摘要

背景

本研究评估了 WNT 家族成员和 RUNX2 的遗传变异是否与颅面成熟有关，从而研究儿童和青少年的牙齿和骨骼成熟度。

方法

使用巴西患者正畸治疗前的射线照片（7 至 17 岁）评估牙齿（全景射线照片）和骨骼成熟度（头颅侧位射线照片）。根据出生日期和拍摄射线照片的时间计算实际年龄（CA）。对于牙齿成熟度分析，采用 Demirjian（1973 年）方法，并计算出牙龄与实际年龄的差值（DA-CA）。对于骨骼成熟度分析，采用 Baccetti 等人（2005 年）的方法，将患者分为“骨骼成熟延迟”、“骨骼成熟提前”或“骨骼成熟正常”。从颊细胞中提取 DNA 用于 WNT 家族基因中的两个遗传变异的基因分型：WNT3A 中的 rs708111（G>A）和 WNT11 中的 rs1533767（G>A）；以及 RUNX2 中的两个遗传变异：rs1200425（G>A）和 rs59983488（G>T）。进行了统计学分析，p 值<0.05 表示存在显著差异。