Madalena Isabela Ribeiro, Reis Caio Luiz Bitencourt, Matsumoto Mirian Aiko Nakane, Stuani Maria Bernadete Sasso, Mattos Natanael Henrique Ribeiro, Oliveira Daniela Silva Barroso de, Oliveira Maria Angélica Hueb de Menezes, Roskamp Liliane, Küchler Erika Calvano, Baratto-Filho Flares
Universidade da Região de Joinville - UNIVILLE, Departamento de Odontologia, Joinville, Santa Catarina, Brasil.
Universidade de Uberaba - UNIUBE, Departamento de Biomateriais, Uberaba, Minas Gerais, Brasil.
J Appl Oral Sci. 2023 Sep 25;31:e20230184. doi: 10.1590/1678-7757-2023-0184. eCollection 2023.
Genetic polymorphisms have been shown to influence several physiological traits, including dental and craniofacial characteristics. Understanding the clinical relevance of genetic polymorphisms in dental practice is crucial to personalize treatment plans and improve treatment outcomes.
to evaluate the association between dental age and genetic polymorphisms in genes encoding estrogen receptors alpha and beta (ESR1 and ESR2, respectively) in a sample of Brazilian children.
This retrospective cross-sectional study was performed with children undergoing orthodontic treatment. Patients with syndromes, congenital anomalies, craniofacial deformities, under hormonal or systemic treatment, and with a previous history of facial trauma were excluded. Panoramic radiographs were used to assess dental age according to the Demirjian, Goldstein, and Tanner method. A delta [dental age-chronological age (DA-CA)] was obtained, which shows whether the patient tends to have a normal, delayed (negative values), or advanced (positive values) dental age. DNA isolated from buccal cells was used to genotype four genetic polymorphisms: rs9340799 (A>G) and rs2234693 (C>T), located in ESR1; and rs1256049 (C>T) and rs4986938 (C>T), located in ESR2. A statistical analysis was performed and values of p<0.05 indicated statistical difference.
A total of 79 patients were included, 44 (55.70%) girls and 35 (44.30%) boys. The Demirjian, Goldstein, and Tanner method, in general, overestimated patients' age by 0.75 years. There was no difference in the delta of dental age between the sexes (p>0.05). Genetic polymorphisms in ESR1 and ESR2 were not associated with dental age (p>0.05).
The studied genetic polymorphisms in ESR1 and ESR2 were not associated with dental age in Brazilian children.
基因多态性已被证明会影响多种生理特征，包括牙齿和颅面特征。了解基因多态性在牙科实践中的临床相关性对于制定个性化治疗方案和改善治疗效果至关重要。
评估巴西儿童样本中牙齿年龄与编码雌激素受体α和β（分别为ESR1和ESR2）的基因中的基因多态性之间的关联。
这项回顾性横断面研究是对接受正畸治疗的儿童进行的。排除患有综合征、先天性异常、颅面畸形、正在接受激素或全身治疗以及有面部创伤史的患者。根据德米尔坚、戈尔茨坦和坦纳方法，使用全景X线片评估牙齿年龄。计算出一个差值[牙齿年龄 - 实际年龄（DA - CA）]，该差值表明患者的牙齿年龄倾向于正常、延迟（负值）还是提前（正值）。从颊细胞中分离出的DNA用于对四种基因多态性进行基因分型：位于ESR1中的rs9340799（A>G）和rs2234693（C>T）；以及位于ESR2中的rs1256049（C>T）和rs4986938（C>T）。进行了统计分析，p<0.05的值表示有统计学差异。
共纳入79例患者，其中女孩44例（55.70%），男孩35例（44.30%）。总体而言，德米尔坚、戈尔茨坦和坦纳方法将患者年龄高估了0.75岁。不同性别之间的牙齿年龄差值无差异（p>0.05）。ESR1和ESR2中的基因多态性与牙齿年龄无关（p>0.05）。
在巴西儿童中，所研究的ESR1和ESR2基因多态性与牙齿年龄无关。
