Li Jumou, Shi Kun, Zhang Zhijun, Xu Jianhe, Yu Huilei
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Sheng Wu Gong Cheng Xue Bao. 2023 Jun 25;39(6):2158-2189. doi: 10.13345/j.cjb.221001.
The synthesis of fine chemicals using multi-enzyme cascade reactions is a recent hot research topic in the field of biocatalysis. The traditional chemical synthesis methods were replaced by constructing multi-enzyme cascades, then the green synthesis of a variety of bifunctional chemicals can be achieved. This article summarizes the construction strategies of different types of multi-enzyme cascade reactions and their characteristics. In addition, the general methods for recruiting enzymes used in cascade reactions, as well as the regeneration of coenzyme such as NAD(P)H or ATP and their application in multi-enzyme cascade reactions are summarized. Finally, we illustrate the application of multi-enzyme cascades in the synthesis of six bifunctional chemicals, including ω-amino fatty acids, alkyl lactams, α, ω-dicarboxylic acids, α, ω-diamines, α, ω-diols, and ω-amino alcohols.
利用多酶级联反应合成精细化学品是生物催化领域最近的一个热门研究课题。通过构建多酶级联反应取代传统化学合成方法，进而实现多种双功能化学品的绿色合成。本文总结了不同类型多酶级联反应的构建策略及其特点。此外，还总结了级联反应中所用酶的招募通用方法，以及辅酶如NAD(P)H或ATP的再生及其在多酶级联反应中的应用。最后，我们阐述了多酶级联反应在六种双功能化学品合成中的应用，包括ω-氨基脂肪酸、烷基内酰胺、α,ω-二羧酸、α,ω-二胺、α,ω-二醇和ω-氨基醇。
