Department of Pharmacognosy and Medicinal Plants, Faculty of Pharmacy (for Girls), Al-Azhar University, P.O. Box 11651, Nasr City, Cairo, Egypt.
Department of Medical Microbiology & Immunology, Faculty of Medicine (for Girls), Al-Azhar University, P.O. Box 11754, Nasr City, Cairo, Egypt.
Z Naturforsch C J Biosci. 2023 Jul 4;78(9-10):365-376. doi: 10.1515/znc-2023-0075. Print 2023 Sep 26.
L. is a medicinal plant that displays valuable pharmacological properties, including antioxidant, antimicrobial, analgesic, antibacterial, antiviral and anti-inflammatory properties. This study targeted to isolate and identify some constituents of leaves using ultra-performance liquid chromatography coupled with mass spectroscopy (UPLC-MS/MS) and different chromatographic techniques. anti-MERS and anti-SARS-CoV-2 activity for different fractions and for two pure isolated compounds, lupeol (RS) and ricinine (RS1) were evaluated using a plaque reduction assay with three different mechanisms and IC based on their cytotoxic concentration (CC) from an MTT assay using Vero E6 cell line. Isolated phytoconstituents and remdesivir are assessed for anti-COVID-19 activity using molecular docking tools. The methylene chloride extract showed pronounced virucidal activity against SARS-CoV-2 (IC = 1.76 μg/ml). It was also shown that ricinine had superior potential activity against SARS-CoV-2, (IC = 2.5 μg/ml). Lupeol displayed the most potency against MERS, (IC = 5.28 μg/ml). Ricinine appeared to be the most biologically active compound. The study showed that and its isolated compounds have potential natural virucidal activity against SARS-COV-2; however, additional exploration is necessary and study for their activity.
L. 是一种药用植物，具有有价值的药理学特性，包括抗氧化、抗菌、镇痛、抗菌、抗病毒和抗炎特性。本研究旨在使用超高效液相色谱与质谱联用（UPLC-MS/MS）和不同的色谱技术分离和鉴定其叶子中的一些成分。使用三种不同机制的蚀斑减少测定法和基于 Vero E6 细胞系 MTT 测定法的细胞毒性浓度 (CC) 的 IC，评估不同馏分和两种分离得到的纯化合物（羽扇豆醇（RS）和瑞香素（RS1））对 MERS 和 SARS-CoV-2 的抗活性。使用分子对接工具评估分离的植物成分和瑞德西韦对 COVID-19 的抗活性。二氯甲烷提取物对 SARS-CoV-2 表现出明显的杀病毒活性（IC = 1.76 μg/ml）。还表明瑞香素对 SARS-CoV-2 具有更高的潜在活性（IC = 2.5 μg/ml）。羽扇豆醇对 MERS 的活性最强（IC = 5.28 μg/ml）。瑞香素似乎是最具生物活性的化合物。研究表明，L.及其分离的化合物对 SARS-CoV-2 具有潜在的天然杀病毒活性；然而，还需要进一步探索和研究其抗活性。
