Instituto de Biología y Medicina Experimental (IBYME), Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina (CONICET), Fundación IBYME (FIBYME), Buenos Aires, Argentina.
Cátedra de Teriogenología, UBA, Facultad de Ciencias Veterinarias, Instituto de Investigación y Tecnología en Reproducción Animal, Buenos Aires, Argentina.
Reprod Domest Anim. 2023 Sep;58(9):1244-1250. doi: 10.1111/rda.14424. Epub 2023 Jul 19.
Glass wool column filtration (GWCF) selects human, bull, boar, dog and buffalo spermatozoa, but reports in the horse are scarce. Single-layer colloid centrifugation with Androcoll-E™ is currently the standard procedure to select good-quality equine sperm. This study was designed to assess GWCF (50 and 75 mg columns; GWCF-50 and GWCF-75, respectively) efficacy to select good-quality sperm from fresh and frozen-thawed equine semen, and to compare its performance with Androcoll-E™ colloid centrifugation. Percentage total motile (TM), progressively motile (PM), morphologically normal (MN), osmotically competent (HOS+) and acrosome-intact/osmotically competent (AI/HOS+) sperm were determined. In studies done with fresh semen samples (n = 17), suspensions subjected to GWCF-50 showed an improvement (p < .05) in PM and HOS+ sperm after selection. With GWCF-75, an increase (p < .05) in PM, MN and HOS+ sperm was observed. Results with GWCF were comparable or better than with Androcoll-E™ selection. Sperm recovery was similar between procedures for all semen parameters. Total sperm count recovery was lower after GWCF-75 (GWCF-50 = 60.0; GWCF-75 = 51.0; Androcoll-E™ = 76.0 million sperm; median; p = .013), but results on total progressive sperm count were similar (GWCF-50 = 23.0; GWCF-75 = 27.0; Androcoll-E™ = 24.0 million sperm; median; p = .3850). Using frozen-thawed semen samples (n = 16), an improvement (p < .05) in TM, PM, NM, HOS+ and AI/HOS+ sperm was observed in GWCF-75 filtrates. Results were comparable to Androcoll-E™ centrifugation, except HOS+ that increased (p < .05) only after GWCF-75. Recovery was comparable for all parameters in frozen samples. GWCF is a simple and low-cost procedure that selects equine sperm with a quality comparable to colloid centrifugation with Androcoll-E™.
玻璃纤维柱过滤法（GWCF）可用于选择人、牛、猪、狗和水牛的精子，但在马中的报道较少。目前，使用 Androcoll-E™的单层胶体离心法是选择优质马精子的标准程序。本研究旨在评估 GWCF（50 和 75mg 柱；GWCF-50 和 GWCF-75）从新鲜和冷冻解冻的马精液中选择优质精子的效果，并将其与 Androcoll-E™胶体离心法进行比较。分别测定总活动精子（TM）、前向运动精子（PM）、形态正常精子（MN）、渗透活力精子（HOS+）和顶体完整/渗透活力精子（AI/HOS+）的百分比。在对新鲜精液样本（n=17）的研究中，经过 GWCF-50 处理的悬浮液在选择后 PM 和 HOS+精子的活力得到改善（p<.05）。使用 GWCF-75 后，PM、MN 和 HOS+精子的活力增加（p<.05）。GWCF 的结果与 Androcoll-E™选择法相当或更好。对于所有精液参数，处理后精子回收率在两种方法之间相似。GWCF-75 后总精子计数回收率较低（GWCF-50=60.0；GWCF-75=51.0；Androcoll-E™=76.0 百万精子；中位数；p=.013），但总前向运动精子计数结果相似（GWCF-50=23.0；GWCF-75=27.0；Androcoll-E™=24.0 百万精子；中位数；p=.3850）。在使用冷冻解冻精液样本（n=16）的研究中，GWCF-75 过滤后的 TM、PM、NM、HOS+和 AI/HOS+精子活力均得到改善（p<.05）。结果与 Androcoll-E™离心法相当，只是 HOS+仅在经过 GWCF-75 后才增加（p<.05）。在冷冻样本中，所有参数的回收率相当。GWCF 是一种简单且低成本的程序，可选择质量与 Androcoll-E™胶体离心法相当的马精子。
