Okumura Taichi, Kitajima Tsumugi, Kaji Takuya, Urano Haruyuki, Matsumoto Kotaro, Inagaki Hideo, Miyamoto Koji, Okada Kazunori, Ueda Minoru
Graduate School of Science, Tohoku University, Aoba-ku, Sendai, Japan.
Graduate School of Life Science, Tohoku University, Aoba-ku, Sendai, Japan.
Biosci Biotechnol Biochem. 2023 Sep 21;87(10):1122-1128. doi: 10.1093/bbb/zbad092.
(3R, 7S)-jasmonoyl-L-isoleucine (JA-Ile) is a lipid-derived plant hormone that regulates plant responses, including biotic/abiotic stress adaptation. In the plant cells, JA-Ile is perceived by COI1-JAZ co-receptor by causing protein-protein interaction between COI1 and JAZ proteins to trigger gene expressions. In this study, we focused on Oryza sativa, a model monocot and an important crop, with 45 possible OsCOI-OsJAZ co-receptor pairs composed of three OsCOI homologs (OsCOI1a, OsCOI1b, and OsCOI2) and 15 OsJAZ homologs. We performed fluorescein anisotropy and pull-down assays to examine the affinity between JA-Ile and OsCOI1a/1b/2-OsJAZ1-15 co-receptor pairs. The results revealed a remarkable difference in the modes of ligand perception by OsCOI1a/1b and OsCOI2. Recently, the unique function of OsCOI2 in some of the JA-responses were revealed. Our current results will lead to the possible development of OsCOI2-selective synthetic ligand.
（3R，7S）-茉莉酰-L-异亮氨酸（JA-Ile）是一种脂质衍生的植物激素，可调节植物的反应，包括生物/非生物胁迫适应性。在植物细胞中，JA-Ile通过引起COI1和JAZ蛋白之间的蛋白质-蛋白质相互作用被COI1-JAZ共受体识别，从而触发基因表达。在本研究中，我们聚焦于单子叶模式植物及重要作物水稻，其由三个OsCOI同源物（OsCOI1a、OsCOI1b和OsCOI2）和15个OsJAZ同源物组成45个可能的OsCOI-OsJAZ共受体对。我们进行了荧光偏振和下拉实验，以检测JA-Ile与OsCOI1a/1b/2-OsJAZ1-15共受体对之间的亲和力。结果揭示了OsCOI1a/1b和OsCOI2在配体识别模式上存在显著差异。最近，OsCOI2在某些JA反应中的独特功能被揭示。我们目前的研究结果将可能促成OsCOI2选择性合成配体的开发。
