Yamamoto M, Takizawa T, Arishima K, Eguchi Y
Anat Rec. 1986 Aug;215(4):361-4. doi: 10.1002/ar.1092150405.
Thyroids from rat fetuses of different ages (from day 14 to day 19 of gestation) were transplanted to organ culture for 2 days, with or without added thyroid-stimulating hormone (TSH) in the medium. Thyroid tissue from 14-day fetuses that initially consisted of irregularly arranged cell cords did not form follicles when cultured in the presence or absence of TSH. Thyroids from 15- and 16-day fetuses initially consisted of epithelial cell masses. When cultured in the presence or absence of TSH they formed follicles, and a majority stored small amounts of colloid. In thyroid transplants from 17-day fetuses, the response to TSH appeared as a significant increase in the follicular diameter and cell height. Thereafter, in all transplants cultured in the presence of TSH, both the follicular diameter and the cell height were markedly greater than in the transplants cultured in the absence of added TSH. These results suggest that the initial formation of thyroid follicles is independent of TSH and that, once developed, follicles become able to respond to TSH.
将不同年龄（妊娠第14天至第19天）大鼠胎儿的甲状腺进行器官培养2天，培养基中添加或不添加促甲状腺激素（TSH）。14天胎儿的甲状腺组织最初由排列不规则的细胞索组成，无论在有无TSH的情况下培养，都不会形成滤泡。15天和16天胎儿的甲状腺最初由上皮细胞团组成。无论在有无TSH的情况下培养，它们都会形成滤泡，并且大多数会储存少量胶体。在17天胎儿的甲状腺移植中，对TSH的反应表现为滤泡直径和细胞高度显著增加。此后，在所有添加TSH培养的移植中，滤泡直径和细胞高度均明显大于未添加TSH培养的移植。这些结果表明，甲状腺滤泡的初始形成独立于TSH，并且一旦发育，滤泡就能够对TSH作出反应。
