Chen Ti, Xu Huan, Yao Xiaoyan, Luo Zhen
Department of Laboratory Medicine, The Third Xiangya Hospital, Central South University, Changsha, China.
Department of Medical Laboratory Science, School of Medicine, Hunan Normal University, Changsha, China.
Front Microbiol. 2023 Jun 19;14:1209358. doi: 10.3389/fmicb.2023.1209358. eCollection 2023.
Glucose is a crucial carbon source for the growth of , but an excess of glucose is detrimental and even leads to cell death. Pyruvate, the central metabolite of glycolysis, has been shown to have anti-inflammatory and antioxidant properties. This study aimed to investigate the protective effect of pyruvate on under high glucose conditions. Sodium pyruvate greatly increased the cytotoxicity of strain BAA-1717 to human erythrocytes and neutrophils . However, the cytotoxicity and survival of were significantly reduced by high glucose, which was restored to normal levels by the addition of sodium pyruvate. The expression of hlg and lukS in was higher in the LB-GP cultures than that in LB-G cultures, but there was no significant difference in cytotoxicity between LB-GP and LB-G cultures. Furthermore, the hemolytic activity of supernatants could be inhibited by the cell-free culture medium (CFCM) of LB-G cultures, suggesting that high levels of extracellular proteases were presence in the CFCM of LB-G cultures, resulting in degradation of the hemolytic factors. The expression of sarA, which negatively regulates extracellular protease secretion, was higher in LB-GP cultures than that in LB-G cultures. Additionally, sodium pyruvate increased acetate production in , which helps maintain cell viability under acidic environment. In conclusion, pyruvate plays an important role in the survival and cytotoxicity of under high glucose conditions. This finding may aid in the development of effective treatments for diabetic foot infections.
葡萄糖是……生长的关键碳源，但过量的葡萄糖是有害的，甚至会导致细胞死亡。丙酮酸作为糖酵解的中心代谢产物，已被证明具有抗炎和抗氧化特性。本研究旨在探讨丙酮酸在高糖条件下对……的保护作用。丙酮酸钠极大地增加了BAA - 1717菌株对人红细胞和中性粒细胞的细胞毒性。然而，高糖显著降低了……的细胞毒性和存活率，添加丙酮酸钠后可恢复至正常水平。在LB - GP培养物中，……中hlg和lukS的表达高于LB - G培养物，但LB - GP和LB - G培养物之间的细胞毒性没有显著差异。此外，LB - G培养物的无细胞培养基（CFCM）可抑制……上清液的溶血活性，这表明LB - G培养物的CFCM中存在高水平的细胞外蛋白酶，导致溶血因子降解。负调控细胞外蛋白酶分泌的sarA在LB - GP培养物中的表达高于LB - G培养物。此外，丙酮酸钠增加了……中乙酸的产生，这有助于在酸性环境下维持细胞活力。总之，丙酮酸在高糖条件下对……的存活和细胞毒性起着重要作用。这一发现可能有助于开发治疗糖尿病足感染的有效方法。
需注意，原文中部分内容缺失关键信息，翻译可能存在一定局限性。
