Jiao Meng-Ying, Zhang Jie, Cheng Wen-Wen, Song Xin, Long Yue-Hong, Xing Zhao-Bin
College of Life Science, North China University of Science and Technology, Tangshan, 063210 Hebei China.
3 Biotech. 2023 Jul;13(7):259. doi: 10.1007/s13205-023-03678-w. Epub 2023 Jul 1.
In this study, through analysis of the genome of () 228 genes were identified and classified into 5 groups AP2 (47 genes), ERF (108 genes), RAV (6 genes), DREB (64 genes), and soloist (3 genes). According to the AP2/ERF classification of , the AP2/ERF proteins were subdivided into 15 groups. The gene structure and motifs of each group of AP2/ERF in were highly similar, which confirmed the conservation of genes. The genes were unevenly distributed on chromosomes, and a total of four pairs of tandem repeats, and 84 co-linear gene pairs were found, so the genes expanded in a fragment replication manner, and dominated by pure selection during evolution. By analyzing the transcriptome data of under different drought stress conditions, 87 genes with differential expression were obtained, of which 10 genes with highly significant differences were further analyzed and screened for qRT-PCR validation. To the best of our knowledge, this is the first report on the gene of , and the bioinformatics analysis and experimental validation provided valuable information about them, which is of great significance for further research on the molecular mechanisms of in response to drought stress.
在本研究中,通过对()基因组的分析,鉴定出228个基因并将其分为5组:AP2(47个基因)、ERF(108个基因)、RAV(6个基因)、DREB(64个基因)和soloist(3个基因)。根据()的AP2/ERF分类,AP2/ERF蛋白被细分为15组。()中每组AP2/ERF的基因结构和基序高度相似,这证实了()基因的保守性。()基因在染色体上分布不均,共发现四对串联重复序列和84对共线性基因对,因此()基因以片段复制的方式扩展,并且在进化过程中以纯化选择为主导。通过分析()在不同干旱胁迫条件下的转录组数据,获得了87个差异表达的()基因,其中对10个差异极显著的基因进行了进一步分析,并通过qRT-PCR验证进行筛选。据我们所知,这是关于()的()基因的首次报道,生物信息学分析和实验验证为它们提供了有价值的信息,这对于进一步研究()响应干旱胁迫的分子机制具有重要意义。