Hao Lidong, Shi Shubing, Guo Haibin, Li Ming, Hu Pan, Wei Yadong, Feng Yanfei
College of Agriculture, Xinjiang Agriculture University, Urumqi, China.
College of Agriculture and Hydraulic Engineering, Sui Hua University, Suihua, China.
PeerJ. 2020 Jul 17;8:e9551. doi: 10.7717/peerj.9551. eCollection 2020.
The Ethylene-Response Factor (ERF) subfamily transcription factors (TFs) belong to the APETALA2/Ethylene-Responsive Factor (AP2/ERF) superfamily and play a vital role in plant growth and development. However, identification and analysis of the ERF subfamily genes in maize have not yet been performed at genome-wide level. In this study, a total of 76 ERF subfamily TFs were identified and were found to be unevenly distributed on the maize chromosomes. These maize ERF (ZmERF) TFs were classified into six groups, namely groups B1 to B6, based on phylogenetic analysis. Synteny analysis showed that 50, 54, and 58 of the genes were orthologous to those in rice, Brachypodium, and Sorghum, respectively. -element analysis showed that elements related to plant growth and development, hormones, and abiotic stress were identified in the promoter region of genes. Expression profiles suggested that genes might participate in plant development and in response to salinity and drought stresses. Our findings lay a foundation and provide clues for understanding the biological functions of ERF TFs in maize.
乙烯响应因子(ERF)亚家族转录因子属于APETALA2/乙烯响应因子(AP2/ERF)超家族,在植物生长发育中起着至关重要的作用。然而,尚未在全基因组水平上对玉米中的ERF亚家族基因进行鉴定和分析。在本研究中,共鉴定出76个ERF亚家族转录因子,发现它们在玉米染色体上分布不均。基于系统发育分析,这些玉米ERF(ZmERF)转录因子被分为六组,即B1至B6组。共线性分析表明,分别有50、54和58个基因与水稻、短柄草和高粱中的基因直系同源。顺式作用元件分析表明,在基因启动子区域鉴定到了与植物生长发育、激素和非生物胁迫相关的元件。表达谱分析表明,基因可能参与植物发育以及对盐度和干旱胁迫的响应。我们的研究结果为理解ERF转录因子在玉米中的生物学功能奠定了基础并提供了线索。