研究报告：一种制备抗牙龈卟啉单胞菌卵黄免疫球蛋白 Y 的新方法。
Research Note: A novel method for preparation of egg yolk immunoglobulin Y against Porphyromonas gingivalis.
机构信息
School of Stomatology, Jinzhou Medical University, Jinzhou, Liaoning 121000, China; The Second Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, China.
School of Stomatology, Jinzhou Medical University, Jinzhou, Liaoning 121000, China.
出版信息
Poult Sci. 2023 Sep;102(9):102863. doi: 10.1016/j.psj.2023.102863. Epub 2023 Jun 11.
Porphyromonas gingivalis (P. gingivalis, P. g) is the main pathogen of periodontal disease, which is treated with egg yolk immunoglobulin Y (IgY) against P. gingivalis. In order to quickly obtain IgY, 30 hens were immunized with inactivated P. gingivalis. The purification of IgY was carried out by the oleic acid (OA) method and the classical method (AS), respectively. The IgY antibody characteristics and antibacterial effects in HPDLF cells were detected by SDS-PAGE, indirect ELISA, Western blot and viability/toxicity assays. SDS-PAGE and Western blot analysis showed that IgY molecules which were rapidly purified by OA method were complete and specific to P. gingivalis. In addition, the results of crystal violet staining and bacterial staining indicated that IgY could agglutinate with P. gingivalis, inhibiting bacterial invasion of host cells. This study is the first to rapidly and efficiently purify IgY by OA method, and the purified IgY is expected to be used in the detection and treatment of P. gingivalis.
牙龈卟啉单胞菌（P. gingivalis，P. g）是牙周病的主要病原体，可使用针对 P. gingivalis 的蛋黄免疫球蛋白 Y（IgY）进行治疗。为了快速获得 IgY，用灭活的 P. gingivalis 对 30 只母鸡进行免疫。分别采用油酸（OA）法和经典法（AS）对 IgY 进行纯化。通过 SDS-PAGE、间接 ELISA、Western blot 和细胞活力/毒性测定检测 HPDLF 细胞中 IgY 抗体特性和抗菌作用。SDS-PAGE 和 Western blot 分析表明，OA 法快速纯化的 IgY 分子完整且特异性针对 P. gingivalis。此外，结晶紫染色和细菌染色的结果表明 IgY 可与 P. gingivalis 凝集，抑制细菌侵入宿主细胞。本研究首次通过 OA 法快速高效地纯化 IgY，预期纯化的 IgY 可用于 P. gingivalis 的检测和治疗。
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