Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, S7N 5B4, Canada; Agriculture and Agri-Food Canada, Saskatoon, SK, S7N 0×2, Canada.
Hy-Line International, Dallas Center, IA, 50063, USA.
Poult Sci. 2023 Sep;102(9):102850. doi: 10.1016/j.psj.2023.102850. Epub 2023 Jun 9.
Gonadal tissue transfer is considered one of the best methods to preserve genetic variability. Poultry hosts can receive a gonad from a donor of a different genetic background, sustain the growth of this graft, and produce gametes from it. Unfortunately, the host's strong immune response may significantly reduce the gonadal graft's ability to reach maturity. Our study aimed to evaluate the influence of MHC-B alleles in rejecting a gonadal graft of similar or different genetic backgrounds. In the first experiment, ovarian tissue was transplanted to chicks of similar genetic backgrounds, either Lohmann White (LW) with variable MHC-B or Barred Rock (BR) with fixed MHC-B. The sustained growth of donor ovarian tissues occurred in (4/7 hosts) BR (MHC-B matched) hosts only-one of these graft-positive-BR hens produced eggs derived from the donor ovary. No grafts were recovered when the host and the donor had an LW background (0/9; MHC-B mismatched). In the second experiment, ovarian transplantation was done between chicks of either similar or different genetic backgrounds (Brown Leghorn [BL], BR, and BL/BR F1). The 2 pure lines contained only one MHC-B allele, whereas the F1 heterozygotes had both. All host birds were given a daily dose of an immunosuppressant (mycophenolate mofetil) until maturity. The success rate was assessed by microsatellite genotype confirmation of donor-derived ovaries plus physiological and histological analyses of ovarian grafts. In this second experiment, 11 out of 43 ovarian hosts laid eggs. However, all fertilized eggs from these hens were derived from the remnant host ovarian tissue, not from the donor ovaries. A necropsy assessment was done on all 43 host birds. Ten donor grafts were recovered from hosts having matched (6 hosts) and mismatched (4 hosts) MHC-B, and none were functional. Interestingly, 6 of them were enclosed by a serous membrane capsule filled with fluid and had various tissue growth. In addition, clusters of immune cells were observed in all recovered donor grafts. Our results demonstrated that genetic background could greatly influence the success of gonadal transfer in chickens.
生殖腺组织移植被认为是保存遗传变异性的最佳方法之一。家禽宿主可以接收来自不同遗传背景的供体的生殖腺，并维持该移植物的生长，并从中产生配子。不幸的是，宿主强烈的免疫反应可能会显著降低生殖腺移植物成熟的能力。我们的研究旨在评估 MHC-B 等位基因在排斥相似或不同遗传背景的生殖腺移植物中的影响。在第一个实验中，将卵巢组织移植到遗传背景相似的小鸡中，这些小鸡要么是具有可变 MHC-B 的 Lohmann White（LW），要么是具有固定 MHC-B 的 Barred Rock（BR）。只有在 MHC-B 匹配（BR）宿主中（4/7 宿主），供体卵巢组织才能持续生长-这些移植阳性-BR 母鸡中的一只产生了来自供体卵巢的鸡蛋。当宿主和供体具有 LW 背景（0/9；MHC-B 不匹配）时，没有移植物被回收。在第二个实验中，在遗传背景相似或不同的小鸡（Brown Leghorn [BL]、BR 和 BL/BR F1）之间进行了卵巢移植。这两个纯系只含有一个 MHC-B 等位基因，而 F1 杂合子则同时含有两个。所有宿主鸟类都给予每日剂量的免疫抑制剂（霉酚酸酯）直到成熟。通过微卫星基因型确认供体衍生的卵巢以及对卵巢移植物的生理和组织学分析来评估成功率。在第二个实验中，43 个卵巢宿主中有 11 个产卵。然而，这些母鸡的所有受精卵均来自残余宿主卵巢组织，而不是供体卵巢。对所有 43 只宿主鸟类进行了尸检评估。从匹配（6 只宿主）和不匹配（4 只宿主）MHC-B 的宿主中回收了 10 个供体移植物，它们均无功能。有趣的是，其中 6 个被充满液体的浆膜囊封闭，并有各种组织生长。此外，在所有回收的供体移植物中都观察到免疫细胞簇。我们的结果表明，遗传背景可能极大地影响鸡生殖腺转移的成功。
