Department of Biophysics, Faculty of Medicine, Mersin University, Mersin, Turkey.
Department of Biophysics, Faculty of Medicine, Mersin University, Mersin, Turkey.
Biochem Biophys Res Commun. 2023 Sep 24;674:53-61. doi: 10.1016/j.bbrc.2023.06.083. Epub 2023 Jun 29.
In recent years, the use of nanotechnology-based methods has become widespread in the treatment of ocular diseases. Silica nanoparticles (SiO NPs) are most common used NPs in medical field due to their physicochemical properties. SiO NPs can easily cross biological membranes and interact with basic biological structures, causing structural and functional changes in cells. In this study, it was aimed to investigate the dose dependent effect of SiO NPs on retinal pigment epithelium (RPE) in vitro using electrobiophysical, biochemical and histological methods. A commercially purchased human RPE (hARPE-19) cell line was used in this study. Cells were divided into four groups as control, 50 μg/mL SiO, 100 μg/mL SiO and 150 μg/mL SiO groups. Cell index, apoptotic activity, cell cycle and oxidative stress markers were measured in all groups. Findings in the present study showed that SiO nanoparticles reduced cell proliferation, increased oxidative stress, apoptosis and arrest in the G/G phase of the cell cycle as dose dependent manner in ARPE-19 cells. In conclusion, SiO exposure can induce cytotoxic effects in RPE cell line. The results of this study provide clues that exposure to SiO nanoparticles may impair visual function and reduce quality of life. However, further studies are needed in this regard.
近年来，基于纳米技术的方法在眼部疾病的治疗中得到了广泛的应用。由于其物理化学性质，硅纳米粒子（SiO NPs）是最常用于医学领域的 NPs。SiO NPs 可以轻易地穿过生物膜并与基本的生物结构相互作用，导致细胞的结构和功能发生变化。在这项研究中，我们旨在使用电生理、生化和组织学方法，研究 SiO NPs 对体外视网膜色素上皮（RPE）的剂量依赖性作用。本研究使用了商业购买的人 RPE（hARPE-19）细胞系。将细胞分为对照组、50μg/mL SiO 组、100μg/mL SiO 组和 150μg/mL SiO 组共 4 组。在所有组中测量细胞指数、凋亡活性、细胞周期和氧化应激标志物。本研究的结果表明，SiO 纳米粒子以剂量依赖的方式降低了 ARPE-19 细胞的增殖，增加了氧化应激、细胞凋亡和细胞周期 G/G 期阻滞。总之，SiO 暴露会在 RPE 细胞系中诱导细胞毒性作用。本研究的结果提供了线索，表明暴露于 SiO 纳米粒子可能会损害视觉功能并降低生活质量。然而，在这方面还需要进一步的研究。
