Shambhavi Hungund Prabhuling, Makwana Pooja, Pradeep Appukuttan Nair R
Seribiotech Research Laboratory, CSB-Kodathi Campus, Bangalore, Karnataka 560035 India.
Biotechnology Division, Central Sericultural Research and Training Institute, Berhampore, West Bengal 742101 India.
3 Biotech. 2023 Aug;13(8):264. doi: 10.1007/s13205-023-03685-x. Epub 2023 Jul 3.
Infection by microsporidian induced appearance of exclusive protein conjugate of 190 kDa in hemocytes of silkworm L (Lepidoptera: Bombycidae). Mass spectrometry of the band showed peptides of low molecular weight 30 kDa lipoprotein (LP30K). Six accessions of LP30K identified from the hemocytes comprised 30 K lipoprotein 1, 30 K protein 1, 2, 6, 7 and 11. Two uncharacterised proteins (UCP) identified from the hemocytes showed 100% similarity with LP30K sequence, altogether showed abundance after the infection. The LP30K accessions H9J4F6 (Q00802), E5EVW2 and the UCP accessions D4QGC0 and D4QGB9 showed presence of glucose binding protein I domain "ADSDVPNDILEEQLYNSIVVADYDSAVEK" that binds with fungal glucans to inhibit infection. However glucose binding protein II domain "TLAPRTDDVLAEQLYMSVVIGEYETAIAK" is absent in LP30K accessions from hemocytes showed loss of DNA sequences encoding the domain. The accessions H9J4F5, H9B440, A7LIK7 and H9B444 showed 92% identity with LP30K protein (NP_001095198.2) however the glucose binding domain I is absent in these accessions suggesting isoform- specific restricted fungal defense activity. Phylogeny tree of the LP30K homologues showed four groups including microvitellogenin and 30 kDa proteins showing functional diversity endorsed with evolutionary diversity. LP30K accessions with glucose binding domain diverged from that without glucose binding domain exemplify co-evolution for domain- dependent functional roles like storage and immune reactions.
The online version contains supplementary material available at 10.1007/s13205-023-03685-x.
微孢子虫感染导致家蚕L（鳞翅目：蚕蛾科）血细胞中出现190 kDa的独特蛋白质共轭物。对该条带进行质谱分析显示有低分子量30 kDa脂蛋白（LP30K）的肽段。从血细胞中鉴定出的6种LP30K包括30K脂蛋白1、30K蛋白1、2、6、7和11。从血细胞中鉴定出的两种未表征蛋白（UCP）与LP30K序列显示出100%的相似性，在感染后总体呈现丰度。LP30K登录号H9J4F6（Q00802）、E5EVW2以及UCP登录号D4QGC0和D4QGB9显示存在葡萄糖结合蛋白I结构域“ADSDVPNDILEEQLYNSIVVADYDSAVEK”，该结构域与真菌葡聚糖结合以抑制感染。然而，血细胞中LP30K登录号缺乏葡萄糖结合蛋白II结构域“TLAPRTDDVLAEQLYMSVVIGEYETAIAK”，显示编码该结构域的DNA序列缺失。登录号H9J4F5、H9B440、A7LIK7和H9B444与LP30K蛋白（NP_001095198.2）显示出92%的同一性，但这些登录号中不存在葡萄糖结合结构域I，表明存在亚型特异性的受限真菌防御活性。LP30K同源物的系统发育树显示包括微卵黄生成素和30 kDa蛋白在内的四个组，显示出功能多样性并得到进化多样性的支持。具有葡萄糖结合结构域的LP30K登录号与没有葡萄糖结合结构域的登录号不同，体现了在储存和免疫反应等依赖结构域的功能作用方面的共同进化。
在线版本包含可在10.1007/s13205-023-03685-x获取的补充材料。
