Nowier Amira M, Darwish Hassan R, Ramadan Sherif I, Othman Othman E
Biotechnology Research Department, Animal Production Research Institute, Agriculture Research Center, Giza, Egypt.
Cell Biology Department, National Research Centre, Giza, Egypt.
Anim Biotechnol. 2023 Dec;34(9):5028-5036. doi: 10.1080/10495398.2023.2223237. Epub 2023 Jul 6.
The objectives of the current study were to identify polymorphism in the prolactin receptor (PRLR) gene among three Egyptian goat breeds (Zaraibi, Damascus, and Barki) and to investigate the association between PRLR genotype, parity, season of kidding, and litter size factors with milk yield and reproductive traits of Zaraibi goats. One hundred and ninety blood samples were collected for DNA extraction, with 110 from Zaraibi, 40 from Barki, and 40 from Damascus breeds. Three genotypes, CC, CT and TT, for the prolactin receptor gene were identified in the 190 DNA samples using restriction fragment length polymorphism and were confirmed by direct sequencing technique. Milk yield during suckling and lactation periods in addition to age at first conception, gestation length, and litter size were determined in 110 Zaraibi goats. The Zaraibi goats recorded the highest heterozygosity (0.495) and the effective number of alleles (1.972). The g.62130C > T SNP showed a significant association ( < 0.01) with suckling, lactation, and total milk yield of Zaraibi goats with the highest values recorded at the third parity. Age at the first conception and gestation length traits were significantly influenced by the kidding season ( < 0.05) with younger age in autumn and shorter length in spring seasons. Milk yield during the suckling period was significantly ( < 0.01) higher in the case of triplets' litter size. The current study showed that litter size and parity played an important role in the amount of Zaraibi goats' milk yield. The g.62130C > T SNP of the PRLR gene may be a useful marker for assisted selection programs to improve goat milk yield during suckling and lactation periods with the heterozygous genotype CT recording the highest values.
本研究的目的是鉴定三个埃及山羊品种（扎拉伊比、大马士革和巴尔基）催乳素受体（PRLR）基因的多态性，并研究PRLR基因型、胎次、产羔季节和产仔数因素与扎拉伊比山羊产奶量和繁殖性状之间的关联。采集了190份血样用于DNA提取，其中110份来自扎拉伊比品种，40份来自巴尔基品种，40份来自大马士革品种。使用限制性片段长度多态性在190份DNA样本中鉴定出催乳素受体基因的三种基因型CC、CT和TT，并通过直接测序技术进行了确认。测定了110只扎拉伊比山羊的哺乳期和泌乳期产奶量，以及初配年龄、妊娠期长度和产仔数。扎拉伊比山羊的杂合度最高（0.495），等位基因有效数为（1.972）。g.62130C>T SNP与扎拉伊比山羊的哺乳期、泌乳期和总产奶量呈显著关联（<0.01），在第三胎时记录到最高值。初配年龄和妊娠期长度性状受产羔季节的显著影响（<0.05），秋季初配年龄较小，春季妊娠期较短。三胞胎产仔数的情况下，哺乳期产奶量显著更高（<0.01）。本研究表明，产仔数和胎次对扎拉伊比山羊的产奶量起着重要作用。PRLR基因的g.62130C>T SNP可能是辅助选择计划的有用标记，用于提高哺乳期和泌乳期山羊的产奶量，杂合基因型CT记录的数值最高。
