Institute of Biology, University of Graz, Universitätsplatz 2, A-8010 Graz, Austria - Research Group Zoology: Biodiversity & Toxicology, Centre for Environmental Sciences, Hasselt University, Agoralaan Gebouw D, 3590 Diepenbeek, Belgium.
Institute of Biology, University of Graz, Universitätsplatz 2, A-8010 Graz, Austria.
Parasite. 2023;30:27. doi: 10.1051/parasite/2023025. Epub 2023 Jul 6.
Polystomatidae is a monogenean family whose representatives infect mainly (semi)-aquatic tetrapods. Species of Sphyranura Wright, 1879 exhibit ectoparasitism on salamander hosts, with molecular work supporting their inclusion within Polystomatidae, at an early diverging, yet unresolved, position in the clade of otherwise endoparasitic polystomatid parasites of batrachian hosts. Records of representatives of Sphyranura are scarce with genetic data only available for S. oligorchis Alvey, 1933. Based on detailed morphological examination and comparison with type material, we identified worms belonging to Sphyranura infecting Oklahoma salamander (Eurycea tynerensis) as S. euryceae Hughes & Moore, 1943. Along with an amended diagnosis of Sphyranura, we provide the first molecular data for S. euryceae in the form of a mitochondrial genome and nuclear (18S, 28S rRNA) markers. Close morphological similarity between the two species of Sphyranura is reflected in low genetic divergence. Mitochondrial level comparison reveals instances of tRNA gene rearrangements in polystomatids. Although the phylogenetic reconstruction supports Sphyranura as early branching in the lineage of polystomatid monogeneans infecting batrachians, certain nodes remain unresolved.
多形目是单殖吸虫科的一个家族，其代表主要感染半水生四足动物。1879 年，斯芬兰ura Wright 的物种表现出对蝾螈宿主的外寄生虫寄生，分子工作支持将其包含在多形目科内，处于早期分化但尚未解决的位置，即在其他内寄生的多形目科寄生虫的分支中。斯芬兰ura 的代表记录很少，只有 S. oligorchis Alvey，1933 的遗传数据。基于详细的形态学检查和与模式材料的比较，我们确定感染俄克拉荷马蝾螈（Eurycea tynerensis）的斯芬兰ura 蠕虫为 S. euryceae Hughes & Moore，1943。除了对斯芬兰ura 的修正诊断外，我们还以线粒体基因组和核（18S、28S rRNA）标记的形式提供了 S. euryceae 的第一个分子数据。两种斯芬兰ura 物种之间的形态相似性反映在遗传分化程度较低。线粒体水平的比较揭示了多形目科中 tRNA 基因重排的实例。尽管系统发育重建支持斯芬兰ura 作为感染两栖动物的多形目科寄生虫的早期分支，但某些节点仍未解决。
