Department of Psychosomatic Medicine, Inner Mongolia Medical University, Huhhot, China.
Inner Mongolia Key Laboratory of Basic Veterinary Science, Inner Mongolia Agriculture University, Hohhot, China.
FASEB J. 2023 Aug;37(8):e23084. doi: 10.1096/fj.202300373R.
Spermatogenesis is a highly organized process by which undifferentiated spermatogonia self-renew and differentiate into spermatocytes and spermatids. The entire developmental process from spermatogonia to sperm occurs within the seminiferous tubules. Spermatogenesis is supported by the close interaction of germ cells with Sertoli cells. In this study, testicular tissues were collected from Hu sheep at 8 timepoints after birth: 0, 30, 90, 180, 270, 360, 540, and 720 days. Immunofluorescence staining and histological analysis were used to explore the development of male germ cells and Sertoli cells in the Hu sheep testes at these timepoints. The changes in seminiferous tubule diameter and male germ cells in the Hu sheep testes at these different developmental stages were analyzed. Then, specific molecular markers were used to study the proliferation and differentiation of spermatogonia, the timepoint of spermatocyte appearance, and the maturation and proliferation of Sertoli cells in the seminiferous tubules. Finally, the formation of the blood-testes barrier was studied using antibodies against the main components of the blood-testes barrier, β-catenin, and ZO-1. These findings not only increased the understanding of the development of the Hu sheep testes, but also laid a solid theoretical foundation for Hu sheep breeding.
精子发生是一个高度组织化的过程，在此过程中，未分化的精原细胞自我更新并分化为精母细胞和精子。从精原细胞到精子的整个发育过程都发生在曲细精管内。精子发生是由生殖细胞与支持细胞的紧密相互作用来支持的。在这项研究中，我们在出生后 8 个时间点从湖羊睾丸组织中收集了样本：0、30、90、180、270、360、540 和 720 天。我们使用免疫荧光染色和组织学分析来探索这些时间点雄性生殖细胞和支持细胞在湖羊睾丸中的发育情况。分析了不同发育阶段曲细精管直径和雄性生殖细胞在湖羊睾丸中的变化。然后，我们使用特定的分子标记物研究了精原细胞的增殖和分化、精母细胞出现的时间点以及曲细精管中支持细胞的成熟和增殖。最后，我们使用抗血睾屏障主要成分β-catenin 和 ZO-1 的抗体研究了血睾屏障的形成。这些发现不仅增加了对湖羊睾丸发育的理解，也为湖羊的繁殖奠定了坚实的理论基础。
