ProteoFormiX, Beerse, Belgium.
Department of Medical Oncology at Institute Jules Bordet, Brussels, Belgium.
Methods Mol Biol. 2023;2688:15-26. doi: 10.1007/978-1-0716-3319-9_2.
We describe an informatics tool for comfortable browsing through highly complex, multi-gigabyte mass spectrometry histochemistry (MSHC) datasets, via clever ion-specific image extraction.The package is developed particularly for the untargeted localization/discovery of biomolecules such as endogenous (neuro)secretory peptides on histological sections of biobanked formaldehyde-fixed paraffin-embedded (FFPE) samples straight from tissue banks.Atmospheric pressure-MALDI-Orbitrap MSHC data of sections through human pituitary adenomas in which two well-known human neuropeptides are detected are used as an example to demonstrate the key features of the novel software, named HistoSnap.
我们描述了一种信息学工具，通过巧妙的离子特异性图像提取，可舒适地浏览高度复杂的、数千兆字节的质谱组织化学（MSHC）数据集。该软件包专为从组织库中直接获取的生物银行固定的福尔马林包埋（FFPE）样本的组织切片中对生物分子（如内源性（神经）分泌肽）进行无目标定位/发现而开发的。使用通过人垂体腺瘤的切片的大气压 MALDI-Orbitrap MSHC 数据作为示例，演示了名为 HistoSnap 的新型软件的关键功能，其中检测到两种已知的人类神经肽。
