将QV600 LLI微流控系统改造用于容纳适合药物吸收和渗透研究的离体胃肠道组织，采用基质辅助激光解吸电离质谱成像（MALDI MSI）和液相色谱-串联质谱（LC-MS/MS）技术。
The Adaptation of the QV600 LLI Milli-Fluidics System to House Ex Vivo Gastrointestinal Tissue Suitable for Drug Absorption and Permeation Studies, Utilizing MALDI MSI and LC-MS/MS.
作者信息
Spencer Chloe E, Duckett Catherine J, Rumbelow Stephen, Clench Malcolm R
机构信息
Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK.
CRODA Inc (B88), New Castle, DE, USA.
出版信息
Methods Mol Biol. 2023;2688:71-82. doi: 10.1007/978-1-0716-3319-9_7.
Careful formulation of pharmaceuticals for oral delivery is essential to ensure that the optimal amount of the active ingredient reaches its intended site of action. This chapter demonstrates how mass spectrometry can be used in conjunction with ex vivo tissue and an adapted milli-fluidics system to carry out a drug absorption study. MALDI MSI is used to visualize the drug within the small intestine tissue from the absorption experimentation. LC-MS/MS is used to complete a mass balance of the experiment and quantify the amount of drug that has permeated through the tissue.
精心配制口服药物对于确保活性成分的最佳剂量到达其预期作用部位至关重要。本章展示了如何将质谱分析与离体组织和适配的微流控系统结合使用，以开展药物吸收研究。基质辅助激光解吸电离质谱成像（MALDI MSI）用于在吸收实验的小肠组织中可视化药物。液相色谱-串联质谱（LC-MS/MS）用于完成实验的质量平衡，并量化透过组织的药物量。
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