Integrated ratiometric luminescence sensing strategy based on encapsulation of guests in heterobinuclear lanthanide coordination polymer nanoparticles for glucose detection in human serum.

Lanthanide coordination polymers (LnCPs) can be used as a host platform to encapsulate functional guest molecules for the construction of integrated sensing platforms. In this work, two guest molecules, rhodamine B (RhB) and glucose oxidase (GOx), were successfully encapsulated in a heterobinuclear lanthanide coordination polymer synthesized by self-assembly of Ce, Tb and adenosine monophosphate (AMP) to form RhB&GOx@AMP-Tb/Ce. Both guest molecules show good storage stability and minimal leakage. The higher catalytic activity and stability of RhB&GOx@AMP-Tb/Ce is obtained due to the confinement effect compared to free GOx. RhB&GOx@AMP-Tb/Ce exhibits superior luminescence based on the internal tandem energy transfer process of the nanoparticles (Ce→Tb→RhB). Glucose can be oxidized in the presence of GOx to form gluconic acid and HO. Subsequently, Ce in the AMP-Tb/Ce host structure can be oxidized by HO to Ce, thereby interrupt the internal energy transfer process and cause ratiometric luminescence response. Benefiting from the synergistic effect, the smart integrated luminescent glucose probe exhibits a wide linear range (0.4-80 μM) and a low detection limit (74.3 nM) with high sensitivity, selectivity and simplicity, enabling the quantitative detection of glucose in human serum. This work describes a good strategy to construct an integrated luminescence sensor based on lanthanide coordination polymers.