Song Hanwu, Li Jun, Zhou Mi, Li Hongwu, Fan Liangliang, Xu Peilun, Shao Shengxi, Li Jingjing, Xu Chengyu, Zhou Wenguang, Qian Jun
Key Laboratory of Poyang Lake Environment and Resource Utilization, Ministry of Education, and School of Resources & Environment, Nanchang University, Nanchang, 330031, China.
Xinjiang Rao River Hydrological and Water Resources Monitoring Center, Shangrao, 334000, China.
Chemosphere. 2023 Oct;337:139416. doi: 10.1016/j.chemosphere.2023.139416. Epub 2023 Jul 4.
Anaerobic digestion piggery effluent (ADPE) shows high chromaticity and ammonium levels, severely inhibiting algal growth. Fungal pretreatment has great potential for decolorization and nutrient removal from wastewater, which coupled with microalgal cultivation may be a reliable strategy for sustainable ADPE resource utilization. In this study, we selected and identified two locally isolated eco-friendly fungal strains for ADPE pretreatment, and fungal culture conditions were optimized for decolorization and ammonium nitrogen (NH-N) removal. Subsequently, the underlying mechanisms of fungal decolorization and nitrogen removal were investigated, and the feasibility of using pretreated ADPE for algal cultivation was explored. The results showed that two fungal strains were identified as Trichoderma harzianum and Trichoderma afroharzianum, respectively, presenting good growth and decolorization performance for ADPE pretreatment. The optimized culture conditions were as follows: 20% ADPE, 8 g L glucose, initial pH 6, 160 rpm, 25-30 °C, and 0.15 g L initial dry-weight. ADPE decolorization was mainly caused by fungal biodegradation of color-related humic substances through manganese peroxidase secretion. The removed nitrogen was completely converted into fungal biomass as nitrogen assimilated, ca. 90% of which was attributed to NH-N removal. The pretreated ADPE significantly improved algal growth and nutrient removal, demonstrating the feasibility of developing an eco-friendly fungi-based pretreatment technology.
厌氧消化猪场废水（ADPE）色度高且铵含量高，严重抑制藻类生长。真菌预处理在废水脱色和养分去除方面具有巨大潜力，与微藻培养相结合可能是ADPE可持续资源利用的可靠策略。在本研究中，我们筛选并鉴定了两种本地分离的生态友好型真菌菌株用于ADPE预处理，并优化了真菌培养条件以实现脱色和去除铵氮（NH-N）。随后，研究了真菌脱色和脱氮的潜在机制，并探讨了使用预处理后的ADPE进行藻类培养的可行性。结果表明，两种真菌菌株分别被鉴定为哈茨木霉和非洲哈茨木霉，对ADPE预处理表现出良好的生长和脱色性能。优化后的培养条件如下：20% ADPE、8 g/L葡萄糖、初始pH 6、160 rpm、25 - 30°C和0.15 g/L初始干重。ADPE脱色主要是由于真菌通过分泌锰过氧化物酶对与颜色相关的腐殖物质进行生物降解所致。去除的氮完全转化为真菌生物量作为氮同化，其中约90%归因于NH-N的去除。预处理后的ADPE显著改善了藻类生长和养分去除，证明了开发一种基于生态友好型真菌的预处理技术的可行性。
