Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Avenida Prof. Moraes Rego, 1235, Cidade Universitária, CEP 50670-901, Recife, PE, Brazil.
Departamento de Biofísica e Radiobiologia, Centro de Biociências, Universidade Federal de Pernambuco, Avenida Prof. Moraes Rego, 1235, Cidade Universitária, CEP 50670-901, Recife, PE, Brazil.
Chem Biodivers. 2023 Aug;20(8):e202300154. doi: 10.1002/cbdv.202300154. Epub 2023 Jul 27.
Schistosomiasis affects about 260 million people worldwide and the search for new schistosomicidal compounds is urgent. In this study we evaluated the in vitro effect of barbatic acid against schistosomulae and young worms of Schistosoma mansoni. The barbatic acid was evaluated through the bioassay of motility and mortality, cellular viability and ultrastructural analysis of juvenile stages through Scanning Electron Microscopy. Barbatic acid showed a schistosomicidal effect against schistosomulae and young worms of S. mansoni after 3 h of exposure. At the end of 24 h, barbatic acid showed 100 %, 89.5 %, 52 % and 28.5 % of lethality for schistosomulae at the concentrations of 200, 100, 50 and 25 μM, respectively. For young worms, barbatic acid showed 100 % and 31.7 % of lethality at the concentrations of 200 and 100 μM, respectively. Motility changes were observed at all sublethal concentrations. There was a significant reduction in the viability of young worms after exposure to barbatic acid at 50, 100 and 200 μM. Extensive damage to the schistosomulae and young worm's tegument, was observed from 50 μM. This report provides data showing the schistosomicidal effect of barbatic acid on schistosomulae and young worms of S. mansoni, causing death, motility changes and ultrastructural damage to worms.
血吸虫病影响全球约 2.6 亿人，因此迫切需要寻找新的杀血吸虫化合物。本研究评估了巴豆酸对曼氏血吸虫尾蚴和幼蚴的体外效应。通过运动和死亡率、细胞活力以及通过扫描电子显微镜对幼体进行超微结构分析的生物测定评估了巴豆酸。巴豆酸对曼氏血吸虫尾蚴和幼蚴具有杀血吸虫作用，暴露 3 小时后即可观察到。24 小时结束时，200、100、50 和 25 μM 浓度下的巴豆酸对尾蚴的致死率分别为 100%、89.5%、52%和 28.5%。对于幼蚴，200 和 100 μM 浓度下的巴豆酸的致死率分别为 100%和 31.7%。在所有亚致死浓度下均观察到运动变化。暴露于 50、100 和 200 μM 巴豆酸后，幼蚴的存活率显著降低。从 50 μM 开始，可观察到尾蚴和幼蚴的表皮广泛受损。本报告提供的数据显示了巴豆酸对曼氏血吸虫尾蚴和幼蚴的杀血吸虫作用，导致虫体死亡、运动变化和超微结构损伤。
