Dalhouse A D, Langford H G, Walsh D, Barnes T
Behav Neurosci. 1986 Aug;100(4):597-602. doi: 10.1037//0735-7044.100.4.597.
Two experiments were conducted in which adrenalectomized male Sprague-Dawley rats were maintained ad lib on distilled water, 3% saline, and sodium-free food. In Experiment 1, 45 rats were given 100, 200, 400, 800, and 1,000 micrograms/kg/day desoxycorticosterone acetate (DOCA) im for 5 days to determine the dose of DOCA that would produce the lowest voluntary saline intake, and 800 micrograms/kg/day was found to produce the nadir in saline intake. In Experiment 2, 40 rats were placed ad lib on distilled water, saline, and sodium-free food as described above, maintained on 800 micrograms/kg/day DOCA, and infused with 4, 25, 100 micrograms/kg/day angiotensin II (A II) or 0.9% saline. The three A II groups showed significant percentage changes in their saline intake above pre-A II levels; the saline control group showed no change in saline intake from pre-A II level. These results are interpreted to demonstrate the production of salt appetite in rats by peripheral administration of physiological doses of angiotensin II.
进行了两项实验，将肾上腺切除的雄性斯普拉格-道利大鼠随意给予蒸馏水、3%盐水和无钠食物。在实验1中，给45只大鼠每天腹腔注射100、200、400、800和1000微克/千克的醋酸脱氧皮质酮（DOCA），持续5天，以确定能使自愿盐水摄入量最低的DOCA剂量，结果发现800微克/千克/天可使盐水摄入量达到最低点。在实验2中，将40只大鼠如上述那样随意给予蒸馏水、盐水和无钠食物，维持每天800微克/千克的DOCA剂量，并分别注射4、25、100微克/千克/天的血管紧张素II（A II）或0.9%盐水。三个A II组的盐水摄入量相对于注射A II前水平有显著的百分比变化；盐水对照组的盐水摄入量与注射A II前水平相比没有变化。这些结果被解释为表明通过外周给予生理剂量的血管紧张素II可使大鼠产生盐食欲。
