Vandal Katherine, Biondic Savana, Canizo Jesica, Petropoulos Sophie
Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Axe Immunopathologie, Montréal, ON, Canada.
Département de Médecine, Université de Montréal, Montréal, ON, Canada.
Methods Mol Biol. 2024;2767:293-305. doi: 10.1007/7651_2023_494.
Single-cell genomics allow the characterization and quantification of molecular heterogeneity from a wide variety of tissues. Here, we describe the manual dissociation and collection of single cells, a method adapted for the characterization of precious small tissues like preimplantation embryos. We also describe the acquisition of mouse embryos by flushing of the oviducts. The cells can then be used in multiple sequencing protocols, for example, Smart-seq2, Smart-seq3, smallseq, and scBSseq.
单细胞基因组学能够对来自多种组织的分子异质性进行表征和定量分析。在此，我们描述了单细胞的手动解离和收集方法，该方法适用于对珍贵的小组织（如植入前胚胎）进行表征。我们还描述了通过冲洗输卵管获取小鼠胚胎的方法。然后，这些细胞可用于多种测序方案，例如Smart-seq2、Smart-seq3、smallseq和scBSseq。
