Department of Animal Science, Texas A&M University, College Station, Texas, USA.
Department of Animal Science, Kleberg Center, Texas A&M University, College Station, Texas, USA.
Biol Reprod. 2023 Sep 12;109(3):309-318. doi: 10.1093/biolre/ioad073.
Progesterone (P4), estradiol (E2), and expression of their receptors (PGR and ESR1, respectively) by cells of the uterus regulate reproductive performance of mammals through effects on secretion and transport of nutrients into the uterine lumen. This study investigated the effect of changes in P4, E2, PGR, and ESR1 on expression of enzymes for the synthesis and secretion of polyamines. Suffolk ewes (n = 13) were synchronized to estrus (Day 0) and then, on either Day 1 (early metestrus), Day 9 (early diestrus), or Day 14 (late diestrus) of the estrous cycle, maternal blood samples were collected, and ewes were euthanized before obtaining uterine samples and uterine flushings. Endometrial expression of MAT2B and SMS mRNAs increased in late diestrus (P < 0.05). Expression of ODC1 and SMOX mRNAs decreased from early metestrus to early diestrus, and expression of ASL mRNA was lower in late diestrus than in early metestrus (P < 0.05). Immunoreactive PAOX, SAT1, and SMS proteins were localized to uterine luminal, superficial glandular, and glandular epithelia, stromal cells, myometrium, and blood vessels. Concentrations of spermidine and spermine in maternal plasma decreased from early metestrus to early diestrus and decreased further in late diestrus (P < 0.05). The abundances of spermidine and spermine in uterine flushings were less in late diestrus than early metestrus (P < 0.05). These results indicate that synthesis and secretion of polyamines are affected by P4 and E2, as well as the expression of PGR and ESR1 in the endometria of cyclic ewes.
孕激素（P4）、雌二醇（E2）及其受体（PGR 和 ESR1）的表达通过影响营养物质向子宫腔的分泌和转运来调节哺乳动物的生殖性能。本研究探讨了 P4、E2、PGR 和 ESR1 变化对多胺合成和分泌酶表达的影响。萨福克母羊（n = 13）同步发情（第 0 天），然后在发情周期的第 1 天（早发情期）、第 9 天（早发情期）或第 14 天（晚发情期）采集母羊血液样本，然后处死母羊，采集子宫样本和子宫冲洗液。子宫内膜 MAT2B 和 SMSmRNA 的表达在发情后期增加（P < 0.05）。ODC1 和 SMOXmRNA 的表达从发情前期到发情期早期减少，ASLmRNA 的表达在发情后期低于发情前期（P < 0.05）。PAOX、SAT1 和 SMS 蛋白的免疫反应性定位于子宫腔、浅层腺和腺上皮、基质细胞、子宫肌层和血管。母血浆中腐胺和精脒的浓度从发情前期到发情期早期减少，发情后期进一步减少（P < 0.05）。发情后期子宫冲洗液中腐胺和精脒的含量低于发情前期（P < 0.05）。这些结果表明，多胺的合成和分泌受 P4 和 E2 以及发情期母羊子宫内膜中 PGR 和 ESR1 的表达影响。
