Simolo K, Korszun Z R, Stucky G, Moffat K, McLendon G, Bunker G
Biochemistry. 1986 Jul 1;25(13):3773-8. doi: 10.1021/bi00361a007.
Metal hybrid hemoglobins, in which Zn(II) replaces Fe(II), have been structurally characterized by extended X-ray absorption structure (EXAFS) studies. Since Zn and Fe have very different K absorption edge energies, the structures of the ligated (Fe) and unligated (Zn) sites could be examined independently within a single molecule that mimics an intermediate ligation state. The observed EXAFS spectra and associated structural parameters are compared among the ligand free (alpha Zn)2(beta Zn)2, half-ligated (alpha FeCO)2(beta Zn)2 and (alpha Zn)2(beta FeCO)2, and fully ligated (alpha FeCO)2(beta FeCO)2 systems.
其中锌（II）取代铁（II）的金属杂合血红蛋白已通过扩展X射线吸收结构（EXAFS）研究进行了结构表征。由于锌和铁具有非常不同的K吸收边能量，因此可以在模拟中间连接状态的单个分子中独立检查连接的（铁）和未连接的（锌）位点的结构。在无配体的（α锌）2（β锌）2、半连接的（α铁-一氧化碳）2（β锌）2和（α锌）2（β铁-一氧化碳）2以及完全连接的（α铁-一氧化碳）2（β铁-一氧化碳）2系统之间比较了观察到的EXAFS光谱和相关的结构参数。
