Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Applied Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Int J Biol Macromol. 2023 Aug 30;247:125718. doi: 10.1016/j.ijbiomac.2023.125718. Epub 2023 Jul 5.
Recently, developing antibacterial wound dressings based on biomaterials display good biocompatibility and the potential to accelerate wound healing. For this aim, we prepared eco-friendly and biodegradable nanofibers (NFs) based on N-(3-sulfopropyl)chitosan/ poly (ε-caprolactone) incorporated by zeolite imidazolate framework-8 nanoparticles (ZIF-8 NPs) and chamomile essential oil (MCEO) via the electrospinning technique for their efficacy as wound dressing scaffolds. Fabricated NFs were characterized and studied for their structural, morphological, mechanical, hydrophilic, and thermal stability properties. The results of scanning electron microscopy (SEM) revealed that adding the ZIF-8 NPs/ MCEO, very slightly influenced the average diameter of NFs (PCL/SPCS (90:10) with 90 ± 32 nm). The developed uniform MCEO-loaded ZIF-8/PCL/SPCS NFs displayed better cytocompatibility, proliferation, and physicochemical properties (e.g. thermal stability and mechanical properties) than neat NFs. The results of cytocompatibility, DAPI (4',6-diamidino-2-phenylindole) staining study, and SEM micrographs demonstrated that formulated NFs had promising adhesion and proliferation against normal human foreskin fibroblasts-2 (HFF-2 cell line). The prepared NFs revealed excellent antibacterial activity against both Staphylococcus aureus and Escherichia coli with inhibition of 32.3 mm and 31.2 mm, respectively. Accordingly, the newly developed antibacterial NFs hold great potential as effective biomaterials for use as an active platform in wound healing applications.
最近，基于生物材料开发抗菌伤口敷料显示出良好的生物相容性，并具有加速伤口愈合的潜力。为此，我们通过静电纺丝技术制备了基于 N-(3-磺丙基)壳聚糖/聚(ε-己内酯)的生态友好且可生物降解的纳米纤维（NFs），其中掺入了沸石咪唑酯骨架-8 纳米粒子（ZIF-8 NPs）和洋甘菊精油（MCEO），以将其作为伤口敷料支架。对制备的 NFs 进行了结构、形态、机械、亲水和热稳定性等性能的表征和研究。扫描电子显微镜（SEM）的结果表明，添加 ZIF-8 NPs/MCEO 后，NFs 的平均直径略有变化（PCL/SPCS（90:10）为 90±32nm）。开发的均匀负载 MCEO 的 ZIF-8/PCL/SPCS NFs 表现出更好的细胞相容性、增殖能力以及物理化学性能（例如热稳定性和机械性能），优于纯 NFs。细胞相容性、DAPI（4',6-二脒基-2-苯基吲哚）染色研究和 SEM 显微照片的结果表明，配方 NFs 对正常的人包皮成纤维细胞-2（HFF-2 细胞系）具有良好的黏附和增殖能力。所制备的 NFs 对金黄色葡萄球菌和大肠杆菌均表现出出色的抗菌活性，抑制直径分别为 32.3mm 和 31.2mm。因此，新开发的抗菌 NFs 作为有效的生物材料在伤口愈合应用中具有很大的潜力。
